{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,14]],"date-time":"2026-01-14T18:37:49Z","timestamp":1768415869174,"version":"3.49.0"},"reference-count":27,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2012,2,15]],"date-time":"2012-02-15T00:00:00Z","timestamp":1329264000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2012,6]]},"abstract":"<jats:p>Finkelstein A, Levy G H, Hui P, Prasad A, Virk R, Chhieng D C, Carling T, Roman S A, Sosa J A, Udelsman R, Theoharis C G &amp; Prasad M L \u2028(2012) <jats:italic>Histopathology<\/jats:italic>\u00a0<jats:bold>60,<\/jats:bold> 1052\u20131059<\/jats:p><jats:p><jats:bold>Papillary thyroid carcinomas with and without <jats:italic>BRAF<\/jats:italic><\/jats:bold>\u200a<jats:bold>V600E<\/jats:bold>\u2003<jats:bold>mutations are morphologically distinct<\/jats:bold><\/jats:p><jats:p><jats:bold>Aims:\u2002<\/jats:bold> The <jats:italic>BRAF<\/jats:italic> V600E mutation resulting in the production of an abnormal BRAF protein has emerged as the most frequent genetic alteration in papillary thyroid carcinomas (PTCs). This study was aimed at identifying distinctive features in tumours with and without the mutation.<\/jats:p><jats:p><jats:bold>Methods and results:\u2002<\/jats:bold> Thirty\u2010four mutation\u2010positive and 22 mutation\u2010negative tumours were identified by single\u2010strand conformation polymorphism of the amplified <jats:italic>BRAF<\/jats:italic> V600E region in the tumour DNA. Mutation\u2010positive tumours were more common in patients older than 45\u2003years (24\/33, <jats:italic>P<\/jats:italic>\u2003=\u20030.05), in classic (23\/30, <jats:italic>P<\/jats:italic>\u2003=\u20030.01), tall cell (4\/5) and oncocytic\/Warthin\u2010like (2\/2) variants of PTC, and in subcapsular sclerosing microcarcinomas (4\/4). In contrast, all 12 follicular variants (<jats:italic>P<\/jats:italic>\u2003&lt;\u20030.0001) and two diffuse sclerosing variants were negative for the mutation. Mutation\u2010positive tumours displayed infiltrative growth (32\/34, <jats:italic>P<\/jats:italic>\u2003=\u20030.02), stromal fibrosis (33\/34, <jats:italic>P<\/jats:italic>\u2003&lt;\u20030.001), psammoma bodies (17\/34, <jats:italic>P<\/jats:italic>\u2003=\u20030.05), plump eosinophilic tumour cells (22\/34, <jats:italic>P<\/jats:italic>\u2003=\u20030.01), and classic fully developed nuclear features of PTC (33\/34, <jats:italic>P<\/jats:italic>\u2003=\u20030.0001). Encapsulation was significantly associated with mutation\u2010negative tumours (15\/22, <jats:italic>P<\/jats:italic>\u2003=\u20030.02).<\/jats:p><jats:p><jats:bold>Conclusions:\u2002<\/jats:bold>  <jats:italic>BRAF<\/jats:italic> V600E mutation\u2010positive and negative PTCs are morphologically different. Recognition of their morphology may help in the selection of appropriate tumours for genetic testing.<\/jats:p>","DOI":"10.1111\/j.1365-2559.2011.04149.x","type":"journal-article","created":{"date-parts":[[2012,2,15]],"date-time":"2012-02-15T14:54:26Z","timestamp":1329317666000},"page":"1052-1059","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":59,"title":["Papillary thyroid carcinomas with and without <i>BRAF<\/i>\u200a V600E mutations are morphologically distinct"],"prefix":"10.1111","volume":"60","author":[{"given":"Alexander","family":"Finkelstein","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gillian 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