{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,12,27]],"date-time":"2023-12-27T01:16:58Z","timestamp":1703639818540},"reference-count":24,"publisher":"Wiley","issue":"6B","license":[{"start":{"date-parts":[[2012,3,6]],"date-time":"2012-03-06T00:00:00Z","timestamp":1330992000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2012,5]]},"abstract":"<jats:p>Hirsch B, Gr\u00fcnbaum M, Wagner F, Bi Y, Lucka L, Du M\u2010Q, Stein H &amp; D\u00fcrkop H<\/jats:p><jats:p>(2012) <jats:italic>Histopathology<\/jats:italic>\u00a0<jats:bold>60,<\/jats:bold> E19\u2013E27<\/jats:p><jats:p><jats:bold>A novel A20 (TNFAIP3) antibody (Ber\u2010A20) can be used to detect unmutated <jats:italic>A20<\/jats:italic> by immunohistology<\/jats:bold><\/jats:p><jats:p><jats:bold>Aims:\u2002<\/jats:bold>  <jats:italic>A20<\/jats:italic> (TNFAIP3) is a nuclear factor\u2010\u03baB (NF\u2010\u03baB)\u2010inducible component of tumour necrosis factor and Toll\u2010like receptor intracellular signal transduction. It negatively regulates NF\u2010\u03baB, and has been identified as a tumour suppressor. Several studies have described <jats:italic>A20<\/jats:italic> inactivation by deletion of the <jats:italic>A20<\/jats:italic> locus at 6q23, inactivating mutations, and\/or methylation of the <jats:italic>A20<\/jats:italic> promoter in various lymphoma entities.<\/jats:p><jats:p><jats:bold>Methods and results:\u2002<\/jats:bold> We generated a monoclonal antibody against the C\u2010terminus of A20 (Ber\u2010A20) and investigated full\u2010length A20 expression of normal lymphoid tissue and lymphomas for the first time. We identified loss of <jats:italic>A20<\/jats:italic> expression in tumour cells of 24% of classical Hodgkin lymphoma, 27% of diffuse large B\u2010cell lymphoma, 20% of chronic lymphocytic leukaemia, 19% of follicular lymphoma, 13% of mantle cell lymphoma and 8% of primary mediastinal B\u2010cell lymphoma cases by immunohistology. Loss of <jats:italic>A20<\/jats:italic> expression rarely occurred in T\u2010cell non\u2010Hodgkin lymphoma.<\/jats:p><jats:p><jats:bold>Conclusions:\u2002<\/jats:bold> Our data are in agreement with cytogenetic and molecular analyses. Among 21 cases of ocular adnexal marginal zone lymphomas with known <jats:italic>A20<\/jats:italic> mutation status, we detected complete absence of A20 expression, whereas cases with wild\u2010type <jats:italic>A20<\/jats:italic> were weakly A20\u2010positive. We demonstrate that A20 loss can be detected by immunohistology with a sensitivity similar to that of complex molecular and genetic methods.<\/jats:p>","DOI":"10.1111\/j.1365-2559.2012.04181.x","type":"journal-article","created":{"date-parts":[[2012,3,6]],"date-time":"2012-03-06T16:55:57Z","timestamp":1331052957000},"update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["A novel A20 (TNFAIP3) antibody (Ber\u2010A20) can be used to detect unmutated <i>A20<\/i> by immunohistology"],"prefix":"10.1111","volume":"60","author":[{"given":"Burkhard","family":"Hirsch","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria","family":"Gr\u00fcnbaum","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Florian","family":"Wagner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yingwen","family":"Bi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lothar","family":"Lucka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ming\u2010Qing","family":"Du","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Harald","family":"Stein","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Horst","family":"D\u00fcrkop","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,3,6]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2010.06.044"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature02794"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni1110"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.289.5488.2350"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20090528"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2008-08-174110"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/path.2466"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2008-12-194852"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature07969"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2009.234"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2009.010157"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.12875"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/path.1351"},{"key":"e_1_2_7_15_2","volume-title":"Current protocols in molecular biology","author":"Ausubel FM","year":"2010"},{"key":"e_1_2_7_16_2","volume-title":"The leukemia\u2010lymphoma cell line facts book","author":"Drexler HG","year":"2001"},{"key":"e_1_2_7_17_2","volume-title":"World Health Organization classification of tumours. 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