{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T00:06:24Z","timestamp":1791331584591,"version":"4.3.1"},"reference-count":38,"publisher":"Wiley","issue":"6B","license":[{"start":{"date-parts":[[2012,3,6]],"date-time":"2012-03-06T00:00:00Z","timestamp":1330992000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2012,5]]},"abstract":"<jats:p>Rajnai H, B\u00f6d\u00f6r C, Balogh Z, Gagyi \u00c9, Csomor J, Kren\u00e1cs T, T\u00f3th E &amp; Matolcsy A<\/jats:p><jats:p>(2012) <jats:italic>Histopathology<\/jats:italic>\u00a0<jats:bold>60,<\/jats:bold> E66\u2013E75<\/jats:p><jats:p><jats:bold>Impact of the reactive microenvironment on the bone marrow involvement of follicular lymphoma<\/jats:bold><\/jats:p><jats:p><jats:bold>Aims:\u2003<\/jats:bold> Follicular lymphoma (FL) is associated with bone marrow (BM) involvement in approximately 40\u201370% of cases. Previous studies have suggested that the immune\u2010microenvironment of FL plays an important role in the clinical behaviour of the disease. To investigate the role of the microenvironment in BM involvement of FL, we performed immunophenotypical analysis of the reactive cell populations in lymph nodes (LN) and corresponding BM of 35 patients with FL. Microenvironment patterns of BM infiltrates were compared to the corresponding features of the LN in cases with BM manifestation, and the LN microenvironment was compared in FL cases with and without BM involvement.<\/jats:p><jats:p><jats:bold>Methods and results:\u2003<\/jats:bold> Automated image\u2010segmentation\u2010based quantitation was performed in whole digital slides of tissue microarrays of formalin\u2010fixed paraffin\u2010embedded tissue biopsies. We found significantly more CD8<jats:sup>+<\/jats:sup> T lymphocytes, forkhead box protein 3 (FoxP3)<jats:sup>+<\/jats:sup> T lymphocytes and CD68<jats:sup>+<\/jats:sup> macrophages and fewer PD1<jats:sup>+<\/jats:sup> T lymphocytes in the BM than in the matching LN samples. Furthermore, we observed significantly fewer CD8<jats:sup>+<\/jats:sup> T cells and CD68<jats:sup>+<\/jats:sup> macrophages in cases involving the BM compared to those localized only to the LNs.<\/jats:p><jats:p><jats:bold>Conclusions:\u2003<\/jats:bold> Our study showed that different tumour cell growth in the LN and BM may generate different microenvironments, and suggested that the reduced number of cytotoxic T lymphocytes and macrophages in LNs favours BM infiltration of neoplastic cells in FL.<\/jats:p>","DOI":"10.1111\/j.1365-2559.2012.04187.x","type":"journal-article","created":{"date-parts":[[2012,3,6]],"date-time":"2012-03-06T16:54:14Z","timestamp":1331052854000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Impact of the reactive microenvironment on the bone marrow involvement of follicular lymphoma"],"prefix":"10.1111","volume":"60","author":[{"given":"Hajnalka","family":"Rajnai","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Csaba","family":"B\u00f6d\u00f6r","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zs\u00f3fia","family":"Balogh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00c9va","family":"Gagyi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Judit","family":"Csomor","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tibor","family":"Kren\u00e1cs","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erika","family":"T\u00f3th","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e1s","family":"Matolcsy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,3,6]]},"reference":[{"key":"e_1_2_8_2_2","first-page":"220","volume-title":"WHO classification of tumours of haematopoietic and lymphoid tissues","author":"Harris NL","year":"2008"},{"key":"e_1_2_8_3_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.3874430"},{"key":"e_1_2_8_4_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.82.21.7439"},{"key":"e_1_2_8_5_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.144.7.2550"},{"key":"e_1_2_8_6_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V82.6.1848.1848"},{"key":"e_1_2_8_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0277-5379(91)90280-Q"},{"key":"e_1_2_8_8_2","first-page":"912","article-title":"CD40 ligand is constitutively expressed in a subset of T cell lymphomas and on the microenvironmental reactive T cells of follicular lymphomas and Hodgkin\u2019s disease","volume":"147","author":"Carbone A","year":"1995","journal-title":"Am. 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