{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,2]],"date-time":"2026-08-02T14:46:08Z","timestamp":1785681968017,"version":"3.56.0"},"reference-count":62,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2012,3,6]],"date-time":"2012-03-06T00:00:00Z","timestamp":1330992000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2012,7]]},"abstract":"<jats:p>Agostinelli C, Paterson J C, Gupta R, Righi S, Sandri F, Piccaluga P P, Bacci F, Sabattini E, Pileri S A &amp; Marafioti T \u2028(2012) <jats:italic>Histopathology<\/jats:italic>\u00a0<jats:bold>61,<\/jats:bold> 33\u201346<\/jats:p><jats:p><jats:bold>Detection of LIM domain only 2 (LMO2) in normal human tissues and haematopoietic and non\u2010haematopoietic tumours using a newly developed rabbit monoclonal antibody<\/jats:bold><\/jats:p><jats:p><jats:bold>Aims:\u2002<\/jats:bold> We describe a new rabbit monoclonal antibody, raised against a fixation\u2010resistant epitope of the transcription regulator LIM domain only 2 (LMO2).<\/jats:p><jats:p><jats:bold>Methods and results:\u2002<\/jats:bold> Lymphoma cell lines and a large series of normal and neoplastic samples were investigated by Western blot and immunohistochemistry. The antibody detected nuclear positivity for the protein, with the exception of a proportion of classical Hodgkin lymphomas (HLs), peripheral T cell lymphomas (PTCLs) and solid tumours that showed granular cytoplasmic staining. In normal lympho\u2010haematopoietic tissues, LMO2 was expressed at different intensities by CD34<jats:sup>+<\/jats:sup> blasts, haematopoietic precursors, germinal centre (GC), mantle and splenic marginal zone B cells. While reactive with only scattered elements in the thymus and nine of 237 PTCLs, the antibody stained 31 of 39 T\u2010acute lymphoblastic lymphoma\/leukaemias (T\u2010ALLs) and the T\u2010ALL\u2010derived human leukaemic cell line, CCRF\u2010CEM. LMO2 was found in 88% of B\u2010acute lymphoblastic lymphoma\/leukaemias (B\u2010ALLs), 5% chronic lymphocytic leukaemias (CLLs) and 14%, 57% and 41% of mantle, follicular and Burkitt lymphomas, respectively. In the setting of diffuse large B cell lymphomas (DLBCLs), LMO2\u2010positivity was related strongly to a GC phenotype. LMO2 was found in 83% primary mediastinal large B cell lymphomas (PMBLs) and 100% nodular lymphocyte predominant Hodgkin lymphomas (NLPHLs), whereas only 10% of classical HLs were stained. Acute and chronic myeloid leukaemias were usually positive.<\/jats:p><jats:p><jats:bold>Conclusions:\u2002<\/jats:bold> The new anti\u2010LMO2 antibody can be applied confidently to routine sections, contributing to the differential diagnosis of several lymphoma subtypes, subtyping of DLBCLs and potential development of innovative therapies.<\/jats:p>","DOI":"10.1111\/j.1365-2559.2012.04198.x","type":"journal-article","created":{"date-parts":[[2012,3,6]],"date-time":"2012-03-06T17:00:22Z","timestamp":1331053222000},"page":"33-46","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":33,"title":["Detection of LIM domain only 2 (LMO2) in normal human tissues and haematopoietic and non\u2010haematopoietic tumours using a newly developed rabbit monoclonal antibody"],"prefix":"10.1111","volume":"61","author":[{"given":"Claudio","family":"Agostinelli","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer C","family":"Paterson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rajeev","family":"Gupta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simona","family":"Righi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Federica","family":"Sandri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pier 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