{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,5]],"date-time":"2026-11-05T13:22:13Z","timestamp":1793884933563,"version":"4.8.0"},"reference-count":27,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2012,5,31]],"date-time":"2012-05-31T00:00:00Z","timestamp":1338422400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2012,10]]},"abstract":"<jats:p>Insuasti\u2010Beltran G, Steidler N L, Kang H &amp; Reichard K K \u2028(2012) <jats:italic>Histopathology<\/jats:italic>\u00a0<jats:bold>61,<\/jats:bold> 694\u2013701<\/jats:p><jats:p><jats:bold>CD34+ megakaryocytes (\u226530%) are associated with megaloblastic anaemia and non\u2010acute myeloid neoplasia<\/jats:bold><\/jats:p><jats:p><jats:bold>Aims:\u2002<\/jats:bold> To evaluate the sensitivity and specificity of CD34 staining of megakaryocytes (MKs), in order to distinguish non\u2010neoplastic and neoplastic bone marrows (BMs).<\/jats:p><jats:p><jats:bold>Methods and results:\u2002<\/jats:bold> Three hundred BMs (120 non\u2010neoplastic and 180 neoplastic) were evaluated for percentage and intensity of CD34 staining of MKs. The selected non\u2010neoplastic cases included anaemia, autoimmune conditions, immune thrombocytopenia (ITP), and staging BMs. The neoplastic cases included myelodysplastic syndromes and\/or myeloproliferative neoplasms (MDS, MPN, MDS\/MPN). Eight per cent of non\u2010neoplastic (9\/120) cases and 13% of neoplastic (24\/180) cases showed \u226530% CD34+ MKs, and these were essentially restricted to cases of megaloblastic anaemia (MBA) and non\u2010acute myeloid neoplasms. The finding of \u226530% CD34+ MKs did not distinguish between categories of non\u2010acute myeloid neoplasms. MDS cases with \u226530% CD34+ MKs had lower platelet counts than cases with &lt;30% (<jats:italic>P<\/jats:italic>\u2003=\u20030.03).<\/jats:p><jats:p><jats:bold>Conclusions:\u2002<\/jats:bold> In complex BM cases, the presence of \u226530% CD34+ MKs constitutes a potentially useful diagnostic tool with which to distinguish non\u2010acute myeloid neoplasms and MBA from non\u2010MBA reactive conditions, for minimal additional cost.<\/jats:p>","DOI":"10.1111\/j.1365-2559.2012.04259.x","type":"journal-article","created":{"date-parts":[[2012,5,31]],"date-time":"2012-05-31T18:38:32Z","timestamp":1338489512000},"page":"694-701","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["CD34+ megakaryocytes (\u226530%) are associated with megaloblastic anaemia and non\u2010acute myeloid neoplasia"],"prefix":"10.1111","volume":"61","author":[{"given":"Giovanni","family":"Insuasti\u2010Beltran","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nichole L","family":"Steidler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huining","family":"Kang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaaren K","family":"Reichard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,5,31]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1089\/scd.1.1993.2.137"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V87.1.1.1"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1385\/IR:34:1:13"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1080\/10739680902941737"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2007-0597"},{"key":"e_1_2_7_7_2","first-page":"1026","article-title":"Identification of CD34+ cells in normal and pathological bone marrow biopsies by QBEND10 monoclonal antibody","volume":"5","author":"Soligo D","year":"1991","journal-title":"Leukemia"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1159\/000101709"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00428-005-0070-8"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1369\/jhc.4A6514.2005"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V80.12.3022.3022"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/stem.140320"},{"key":"e_1_2_7_13_2","first-page":"171","article-title":"CD34 expression in megakaryocytes favors myelodysplasia","volume":"14","author":"Machhi J","year":"2001","journal-title":"Mod. 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