{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T05:06:11Z","timestamp":1789189571603,"version":"build-2803163510"},"reference-count":10,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2012,11,21]],"date-time":"2012-11-21T00:00:00Z","timestamp":1353456000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:p>Mationg\u2010Kalaw E, Tan L H C, Tay K, Lim S T, Tang T, Lee Y Y L &amp; Tan S Y \u2028(2012) <jats:italic>Histopathology<\/jats:italic>\u2028<jats:bold>Does the proliferation fraction help identify mature B cell lymphomas with double\u2010 and triple\u2010hit translocations?<\/jats:bold><\/jats:p><jats:p><jats:bold>Aims:\u2002<\/jats:bold> The entity \u2018B cell lymphoma, unclassifiable, with features intermediate between diffuse large B cell lymphoma (DLBCL) and Burkitt lymphoma (BL)\u2019 refers to B cell neoplasms that share overlapping characteristics of BL and DLBCL. A subset of these \u2018grey\u2010zone lymphomas\u2019 possesses <jats:italic>C\u2010MYC<\/jats:italic> and <jats:italic>IGH<\/jats:italic> translocations but, in addition, contains additional rearrangements of <jats:italic>BCL2<\/jats:italic> and\/or <jats:italic>BCL6<\/jats:italic> genes. The aim of this study was to investigate if the proliferation fraction by Ki67 immunostaining can be used to identify such double\u2010\/triple\u2010hit lymphomas.<\/jats:p><jats:p><jats:bold>Methods and results:\u2002<\/jats:bold> We studied 492 cases of mature aggressive B cell neoplasms by histology, immunohistochemistry and interphase fluorescence <jats:italic>in\u2010situ<\/jats:italic> hybridization (FISH) using break\u2010apart probes against <jats:italic>C\u2010MYC<\/jats:italic>, <jats:italic>BCL2<\/jats:italic>, <jats:italic>BCL6<\/jats:italic>, <jats:italic>IGH<\/jats:italic>, <jats:italic>MALT1<\/jats:italic>, <jats:italic>PAX5<\/jats:italic> and <jats:italic>CCND1<\/jats:italic>. Forty Burkitt lymphomas and 28 cases of <jats:italic>MYC<\/jats:italic><jats:sup><jats:italic>+<\/jats:italic><\/jats:sup> double\u2010\/triple\u2010hit lymphomas were identified. Of the latter, 77% and 54% displayed proliferation fractions exceeding 75% and 90%, respectively. With a cut\u2010off of &gt;75% by Ki67 immunostaining, the sensitivity and specificity for detection of <jats:italic>MYC<\/jats:italic><jats:sup><jats:italic>+<\/jats:italic><\/jats:sup> double\/triple translocations was 0.77 and 0.36. Raising the proliferation fraction criterion to &gt;90% improved the specificity to 0.62 at the expense of a low sensitivity of 0.54.<\/jats:p><jats:p><jats:bold>Conclusions:\u2002<\/jats:bold> Immunostaining for Ki67 is not a useful approach to prescreen B cell lymphomas for <jats:italic>MYC<\/jats:italic><jats:sup><jats:italic>+<\/jats:italic><\/jats:sup> double\/triple translocations.<\/jats:p>","DOI":"10.1111\/j.1365-2559.2012.04351.x","type":"journal-article","created":{"date-parts":[[2012,6,18]],"date-time":"2012-06-18T19:22:13Z","timestamp":1340047333000},"page":"1214-1218","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Does the proliferation fraction help identify mature B cell lymphomas with double\u2010 and triple\u2010hit translocations?"],"prefix":"10.1111","volume":"61","author":[{"given":"Emarene","family":"Mationg\u2010Kalaw","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leonard H C","family":"Tan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin","family":"Tay","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soon Thye","family":"Lim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tiffany","family":"Tang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yvonne Y L","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soo Yong","family":"Tan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,11,21]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V84.5.1361.1361"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2404529"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.pas.0000168176.71405.e5"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa055351"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1186\/1476-4598-6-35"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02983534"},{"key":"e_1_2_7_8_2","first-page":"233","volume-title":"WHO classification of tumours of haematopoietic and lymphoid tissues","author":"Stein HWR","year":"2008"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.13071"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2003-05-1545"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/path.1218"}],"container-title":["Histopathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1365-2559.2012.04351.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1365-2559.2012.04351.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,15]],"date-time":"2023-10-15T01:58:01Z","timestamp":1697335081000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1365-2559.2012.04351.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,11,21]]},"references-count":10,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2012,12]]}},"alternative-id":["10.1111\/j.1365-2559.2012.04351.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1365-2559.2012.04351.x","archive":["Portico"],"relation":{},"ISSN":["0309-0167","1365-2559"],"issn-type":[{"value":"0309-0167","type":"print"},{"value":"1365-2559","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,11,21]]},"assertion":[{"value":"2012-11-21","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}