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The DC\u2010induced Treg cells are a mixture of CD25<jats:sup>+<\/jats:sup> (10\u201320%) and CD25<jats:sup>\u2212<\/jats:sup> (80\u201390%) T cells. However, all the suppressor activity <jats:italic>in vitro<\/jats:italic> and <jats:italic>in vivo<\/jats:italic> resides in the CD25<jats:sup>+<\/jats:sup> T\u2010cell subset. The CD25<jats:sup>+<\/jats:sup> DC\u2010induced Treg cells can inhibit enteroantigen\u2010induced proliferation <jats:italic>in vitro<\/jats:italic> through a transwell membrane, and their function does not appear to depend on previous activation. DC\u2010induced CD25<jats:sup>+<\/jats:sup> Treg cells display a na\u00efve phenotype, expressing high levels of CD45RB and <jats:sc>l<\/jats:sc>\u2010selectin (CD62L). In addition, the DC\u2010induced Treg cells mediate a stronger suppressive activity than prototype CD25<jats:sup>+<\/jats:sup> regulatory T cells. The DC\u2010induced Treg cells, and hereof purified CD25<jats:sup>+<\/jats:sup> and CD25<jats:sup>\u2212<\/jats:sup> T\u2010cell fractions, were co\u2010injected into severe combined immunodeficiency (SCID) mice with colitis\u2010inducing CD4<jats:sup>+<\/jats:sup>\u2003CD25<jats:sup>\u2212<\/jats:sup> T cells. Both unfractionated CD4<jats:sup>+<\/jats:sup> and purified CD25<jats:sup>+<\/jats:sup> Treg cells fully protected the recipients against the development of colitis. In contrast, co\u2010transfer of fractionated CD25<jats:sup>\u2212<\/jats:sup> T cells offered no protection against disease development. Enterobacterial antigen\u2010exposed CD4<jats:sup>+<\/jats:sup> T cells of the protected mice secreted higher levels of interleukin\u201010 and lower levels of interferon\u2010\u03b3 than the unprotected mice. The present data demonstrate DC\u2010induced CD4<jats:sup>+<\/jats:sup>\u2003CD25<jats:sup>+<\/jats:sup> Treg cells, which phenotypically and functionally differ from the generally accepted prototype of CD25<jats:sup>+<\/jats:sup> Treg cells. These data may initiate new procedures for the expansion of Treg cells for clinical use.<\/jats:p>","DOI":"10.1111\/j.1365-2567.2004.01977.x","type":"journal-article","created":{"date-parts":[[2004,11,18]],"date-time":"2004-11-18T15:38:42Z","timestamp":1100792322000},"page":"499-508","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Characterization of T\u2010regulatory cells, induced by immature dendritic cells, which inhibit enteroantigen\u2010reactive colitis\u2010inducing T\u2010cell responses in vitro and in vivo"],"prefix":"10.1111","volume":"113","author":[{"given":"Monika","family":"Gad","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nanna 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