{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T13:43:33Z","timestamp":1784641413935,"version":"3.55.0"},"reference-count":43,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,4,27]],"date-time":"2005-04-27T00:00:00Z","timestamp":1114560000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunology"],"published-print":{"date-parts":[[2005,7]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>The inhibitory effect on antibody production by immune complexes has been shown to depend on co\u2010ligation of the B\u2010cell antigen receptor (BCR) with the low\u2010affinity receptor for immunoglobulin G (IgG) (Fc\u03b3RIIb, CD32). Here we report that immunoglobulin E (IgE) synthesis, induced in a BCR\u2010independent manner by interleukin\u20104 (IL\u20104) and anti\u2010CD40 antibody, was inhibited by CD32 ligation. The observed effect was specific for CD32 as, first, antibodies directed against other B\u2010cell surface structures had no inhibitory effect, and, second, treatment with anti\u2010CD32 of cells that had been in culture for 2\u2003days was ineffective owing to the down\u2010regulation of CD32 expression. IgE inhibition was also observed in cells stimulated by IL\u20104\/CD40 F(ab\u2032)<jats:sub>2<\/jats:sub> or IL\u20104 plus soluble CD40 ligand, demonstrating that co\u2010cross\u2010linking of CD32 and CD40 was not necessary to induce inhibition. Mechanistic studies into the IgE class switch process demonstrated that IL\u20104\/anti\u2010CD40\u2010induced IgE germline gene transcription and B\u2010cell proliferation were not affected by CD32 ligation. The data demonstrate that the negative regulatory role of the CD32 molecule is not restricted to BCR\u2010induced B\u2010cell activation, but is also functional on other B\u2010cell activation pathways mediated by CD40 and IL\u20104.<\/jats:p>","DOI":"10.1111\/j.1365-2567.2005.02162.x","type":"journal-article","created":{"date-parts":[[2005,6,9]],"date-time":"2005-06-09T10:34:58Z","timestamp":1118313298000},"page":"407-415","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Inhibition of immunoglobulin E synthesis through Fc\u03b3RII (CD32) by a mechanism independent of B\u2010cell receptor co\u2010cross\u2010linking"],"prefix":"10.1111","volume":"115","author":[{"given":"Jutta","family":"Horejs\u2010Hoeck","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Hren","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Geert C.","family":"Mudde","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maximilian","family":"Woisetschl\u00e4ger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,6,9]]},"reference":[{"key":"e_1_2_7_2_2","first-page":"343","article-title":"Regulation and targeting of T\u2010cell immune responses by IgE and IgG antibodies","volume":"86","author":"Bheekha Escura R","year":"1995","journal-title":"Immunology"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-5699(95)80005-0"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/icb.1996.23"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.15.1.203"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.iy.09.040191.002325"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.2139735"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/5.3.239"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.7.2240"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.170.4.1369"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830250143"},{"key":"e_1_2_7_12_2","doi-asserted-by":"crossref","first-page":"574","DOI":"10.4049\/jimmunol.152.2.574","article-title":"Functional analysis of human Fc gamma RII (CD32) isoforms expressed in B lymphocytes","volume":"152","author":"Van Den Herik\u2010Oudijk IE","year":"1994","journal-title":"J Immunol"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2776(08)60465-4"},{"key":"e_1_2_7_14_2","first-page":"967","article-title":"Regulation of the immune response. 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