{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,28]],"date-time":"2026-03-28T10:37:57Z","timestamp":1774694277777,"version":"3.50.1"},"reference-count":28,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2006,5,19]],"date-time":"2006-05-19T00:00:00Z","timestamp":1147996800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunology"],"published-print":{"date-parts":[[2006,7]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Polysorbate 80 (PS80 or Tween\u201080) is often used as an additive to promote the rapid solubilization of pharmaceuticals in aqueous solutions. We investigated whether coinjection of a minimal amount of PS80 had a modulatory effect on the immunotherapeutic effects of Cry (Cryptomeria)\u2010consensus peptide, a novel peptide developed for the therapeutic management of Japanese cedar pollinosis, using a Cry j 1\u2010sensitized mouse model with experimental allergic rhinitis. Subcutaneous challenge with Cry\u2010consensus peptide plus 50\u2003\u00b5g\/ml of PS80 did not affect the antigen\u2010specific proliferation of splenocytes, but decreased the potency of Cry\u2010consensus peptide to inhibit antigen\u2010specific interleukin (IL)\u20105 production by the cells significantly in comparison with challenge with Cry\u2010consensus peptide alone. However, there was no significant difference between the effect of Cry\u2010consensus peptide administration on interferon (IFN)\u2010\u03b3 production in the presence and absence of PS80, indicating that PS80 interfered with the T helper 1 (Th1)\u2010dominant T helper balance induced by Cry\u2010consensus peptide challenge. Moreover, the increase in the level of antigen\u2010specific immunoglobulin G2a (IgG2a) induced by Cry\u2010consensus peptide challenge was inhibited slightly but unambiguously by PS80 coinjection. These <jats:italic>in vitro<\/jats:italic> experiments indicated that PS80 induces Th2\u2010type differentiation of T helper cells through preferential inhibition of IFN\u2010\u03b3 expression relative to IL\u20105 expression in splenocytes in a concentration\u2010dependent manner. In na\u00efve mice, sensitization by Cry\u2010consensus peptide with PS80 induced antigen\u2010specific IL\u20105 production more potently than sensitization by Cry\u2010consensus peptide alone, and when PS80 was added to bone marrow\u2010derived dendritic cells, the endocytosis of fluorescence\u2010labelled Cry\u2010consensus peptide was dramatically inhibited in a concentration\u2010dependent manner. Therefore, we conclude that PS80 has an immunomodulatory effect on the antigen\u2010specific response resulting in a shift towards Th2 predominance with respect to the antigen recognition stage. Taken together, our findings suggest that PS80 might decrease the efficacy of Cry\u2010consensus peptide through modulation of the efficiency of antigen endocytosis and\/or of the direction of successive T helper cell differentiation.<\/jats:p>","DOI":"10.1111\/j.1365-2567.2006.02390.x","type":"journal-article","created":{"date-parts":[[2006,5,19]],"date-time":"2006-05-19T09:34:36Z","timestamp":1148031276000},"page":"392-401","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["PS80 interferes with the antiallergic effect of Cry\u2010consensus peptide, a novel recombinant peptide for immunotherapy of Japanese cedar pollinosis, at very low concentration through modulation of Th1\/Th2 balance"],"prefix":"10.1111","volume":"118","author":[{"given":"Daisuke","family":"Kozutsumi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masako","family":"Tsunematsu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Taketo","family":"Yamaji","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rika","family":"Murakami","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Minehiko","family":"Yokoyama","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kohsuke","family":"Kino","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,5,26]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(84)92805-8"},{"key":"e_1_2_7_3_2","first-page":"234","article-title":"Prevalence of atopic disorders among adolescents in Turku","volume":"47","author":"Varjonen E","year":"1992","journal-title":"Finland Allergy"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1159\/000235067"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0091-6749(83)90550-X"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0091-6749(92)90098-M"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.178.5.1783"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.16.7608"},{"key":"e_1_2_7_9_2","first-page":"190","article-title":"Inhibition of T cell responses by feeding peptides containing major and cryptic epitopes: studies with the Der p.I allergen","volume":"83","author":"Hoyne GF","year":"1994","journal-title":"Immunology"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/0161-5890(94)90090-6"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.1994.tb02272.x"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-5699(96)80606-2"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.275.5296.77"},{"key":"e_1_2_7_14_2","doi-asserted-by":"crossref","first-page":"448","DOI":"10.4049\/jimmunol.161.1.448","article-title":"T cell epitopes in Japanese cedar (Cryptomeria japonica) pollen allergens. Choice of major T cell epitopes in Cry j 1 and Cry j 2 toward design of the peptide\u2010based immunotherapeutics for the management of Japanese cedar pollinosis","volume":"161","author":"Sone T","year":"1998","journal-title":"J Immunol"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1159\/000024194"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3835(99)00355-9"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0928-0987(02)00055-6"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02976689"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11095-004-7684-4"},{"key":"e_1_2_7_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneumeth.2004.02.016"},{"key":"e_1_2_7_21_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.176.6.1693"},{"key":"e_1_2_7_22_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2567.1997.00218.x"},{"key":"e_1_2_7_23_2","doi-asserted-by":"publisher","DOI":"10.1021\/js960483y"},{"key":"e_1_2_7_24_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1016033702220"},{"key":"e_1_2_7_25_2","first-page":"173","article-title":"Tween 80 and endothelium: functional due to tissue damage","volume":"91","author":"Uluoglu C","year":"1996","journal-title":"Res Commun Mol Pathol Pharmacol"},{"key":"e_1_2_7_26_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M502849200"},{"key":"e_1_2_7_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymthe.2004.10.007"},{"key":"e_1_2_7_28_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200324340"},{"key":"e_1_2_7_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tox.2004.02.017"}],"container-title":["Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1365-2567.2006.02390.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1365-2567.2006.02390.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,14]],"date-time":"2023-10-14T00:32:17Z","timestamp":1697243537000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1365-2567.2006.02390.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,5,26]]},"references-count":28,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2006,7]]}},"alternative-id":["10.1111\/j.1365-2567.2006.02390.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1365-2567.2006.02390.x","archive":["Portico"],"relation":{},"ISSN":["0019-2805","1365-2567"],"issn-type":[{"value":"0019-2805","type":"print"},{"value":"1365-2567","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,5,26]]},"assertion":[{"value":"2006-05-26","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}