{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T20:19:05Z","timestamp":1781295545762,"version":"3.54.1"},"reference-count":51,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2006,7,20]],"date-time":"2006-07-20T00:00:00Z","timestamp":1153353600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunology"],"published-print":{"date-parts":[[2006,11]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Primary immunization of infants with protein\u2013polysaccharide conjugate vaccines induces antipolysaccharide antibody and is highly effective in preventing invasive disease caused by encapsulated bacteria. However, recent experience from the UK indicates that this immunity is not sustained in the absence of booster doses of vaccine. This study aimed to establish the kinetics and phenotype of B\u2010cell subpopulations responding to booster immunization with a heptavalent pneumococcal conjugate vaccine (Pnc7), which is to be introduced into the primary immunization schedule in the UK during 2006. Six adult volunteers received a booster dose of Pnc7 12\u201318\u2003months after primary immunization. CD27<jats:sup>hi<\/jats:sup> CD38<jats:sup>hi<\/jats:sup>\u2003CD20<jats:sup>+\/\u2013<\/jats:sup> IgG antibody\u2010forming cells were detected in peripheral blood with maximum frequency at days 6\u20137 after immunization. This was accompanied by a more prolonged rise in memory B cells that required <jats:italic>in vitro<\/jats:italic> stimulation with <jats:italic>Staphylococcus aureus<\/jats:italic> Cowan strain and interleukin\u20102 to induce antibody secretion. These data provide evidence for at least two subsets of antibody\u2010forming cells involved in the secondary humoral response to a glycoconjugate vaccine in primed individuals. A briefly circulating subset of B cells that spontaneously secrete immunoglobulin G may be responsible for early defence against re\u2010encountered encapsulated bacteria. However, the kinetics of the appearance of these cells may indicate that the humoral immune response is too slow in defence against an organism that invades within days of acquisition. The more sustained presence of a memory population may provide persistence of antipolysaccharide antibody after a booster dose of vaccine and may also include re\u2010circulatory populations responsible for further anamnestic responses.<\/jats:p>","DOI":"10.1111\/j.1365-2567.2006.02436.x","type":"journal-article","created":{"date-parts":[[2006,7,20]],"date-time":"2006-07-20T07:42:10Z","timestamp":1153381330000},"page":"328-337","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":50,"title":["The kinetics and phenotype of the human B\u2010cell response following immunization with a heptavalent pneumococcal\u2010CRM<sub>197<\/sub> conjugate vaccine"],"prefix":"10.1111","volume":"119","author":[{"given":"Elizabeth A.","family":"Clutterbuck","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Penny","family":"Salt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah","family":"Oh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arnaud","family":"Marchant","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Beverley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew John","family":"Pollard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,8,3]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(99)00122-X"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2004-01-0346"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.172.1.532"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/s281-001-8166-x"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.inf.0000129685.04847.94"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.284.18.2334"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1086\/376997"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(04)16725-1"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2003.12.015"},{"key":"e_1_2_7_11_2","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1128\/CDLI.5.2.199-204.1998","article-title":"Evaluation of previously assigned antibody concentrations in pneumococcal polysaccharide reference serum; 89SF:by the method of cross\u2010standardization","volume":"5","author":"Concepcion N","year":"1998","journal-title":"Clin Diagn Lab Immunol"},{"key":"e_1_2_7_12_2","first-page":"514","article-title":"Enzyme\u2010linked immunosorbent assay for quantitation of human antibodies to pneumococcal polysaccharides","volume":"10","author":"Wernette CM","year":"2003","journal-title":"Clin Diagn Laboratory Immunol"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3083.1987.tb01047.x"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830200320"},{"key":"e_1_2_7_15_2","first-page":"227","article-title":"Characterization of the cellular immune response following in vivo immunization with pneumococcal polysaccharides in man","volume":"19","author":"Kehrl JH","year":"1983","journal-title":"Bull Eur Physiopathol Respir"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/171.1.198"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/0008-8749(83)90295-2"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(97)00162-X"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2004-07-2507"},{"key":"e_1_2_7_20_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni1016"},{"key":"e_1_2_7_21_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.146.12.4071"},{"key":"e_1_2_7_22_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.23.021704.115732"},{"key":"e_1_2_7_23_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI113304"},{"key":"e_1_2_7_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(01)00328-0"},{"key":"e_1_2_7_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2003.10.018"},{"key":"e_1_2_7_26_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.171.4.1684"},{"key":"e_1_2_7_27_2","doi-asserted-by":"crossref","first-page":"3700","DOI":"10.4049\/jimmunol.148.12.3700","article-title":"IgM and IgG but not cytokine secretion is restricted to the CD27+ B lymphocyte subset","volume":"148","author":"Maurer D","year":"1992","journal-title":"J Immunol"},{"key":"e_1_2_7_28_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V99.2.567"},{"key":"e_1_2_7_29_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830120611"},{"key":"e_1_2_7_30_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1076071"},{"key":"e_1_2_7_31_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20050218"},{"key":"e_1_2_7_32_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri1203"},{"key":"e_1_2_7_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.smim.2004.08.013"},{"key":"e_1_2_7_34_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.23.021704.115723"},{"key":"e_1_2_7_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0952-7915(98)80162-3"},{"key":"e_1_2_7_36_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.0300-9475.2004.01460.x"},{"key":"e_1_2_7_37_2","doi-asserted-by":"publisher","DOI":"10.1086\/374624"},{"key":"e_1_2_7_38_2","doi-asserted-by":"publisher","DOI":"10.1086\/423820"},{"key":"e_1_2_7_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(05)70083-9"},{"key":"e_1_2_7_40_2","doi-asserted-by":"publisher","DOI":"10.1097\/00006454-200211000-00007"},{"key":"e_1_2_7_41_2","doi-asserted-by":"crossref","first-page":"2652","DOI":"10.4049\/jimmunol.159.6.2652","article-title":"CD154\/CD40 and CD70\/CD27 interactions have different and sequential functions in T cell\u2010dependent B cell responses: enhancement of plasma cell differentiation by CD27 signaling","volume":"159","author":"Jacquot S","year":"1997","journal-title":"J Immunol"},{"key":"e_1_2_7_42_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.2002.3406_5.x"},{"key":"e_1_2_7_43_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-065X.2003.00055.x"},{"key":"e_1_2_7_44_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2003.tb06044.x"},{"key":"e_1_2_7_45_2","doi-asserted-by":"crossref","first-page":"7198","DOI":"10.4049\/jimmunol.162.12.7198","article-title":"IgMhigh CD21high lymphocytes enriched in the splenic marginal zone generate effector cells more rapidly than the bulk of follicular B cells","volume":"162","author":"Oliver AM","year":"1999","journal-title":"J Immunol"},{"key":"e_1_2_7_46_2","doi-asserted-by":"publisher","DOI":"10.1002\/1521-4141(200101)31:1<243::AID-IMMU243>3.0.CO;2-0"},{"key":"e_1_2_7_47_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2004.11.004"},{"key":"e_1_2_7_48_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.1.369-377.2005"},{"key":"e_1_2_7_49_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2003.03.001"},{"key":"e_1_2_7_50_2","doi-asserted-by":"publisher","DOI":"10.1097\/00006454-199909000-00004"},{"key":"e_1_2_7_51_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(00)00314-5"},{"key":"e_1_2_7_52_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.169.11.6149"}],"container-title":["Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1365-2567.2006.02436.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1365-2567.2006.02436.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,13]],"date-time":"2023-10-13T18:32:17Z","timestamp":1697221937000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1365-2567.2006.02436.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,8,3]]},"references-count":51,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2006,11]]}},"alternative-id":["10.1111\/j.1365-2567.2006.02436.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1365-2567.2006.02436.x","archive":["Portico"],"relation":{},"ISSN":["0019-2805","1365-2567"],"issn-type":[{"value":"0019-2805","type":"print"},{"value":"1365-2567","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,8,3]]},"assertion":[{"value":"2006-08-03","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}