{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T14:40:03Z","timestamp":1761662403916},"reference-count":56,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2006,8,18]],"date-time":"2006-08-18T00:00:00Z","timestamp":1155859200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunology"],"published-print":{"date-parts":[[2006,11]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>We mapped mouse CD4 T\u2010cell epitopes located in three structurally distinct regions of the V antigen of <jats:italic>Yersinia pestis<\/jats:italic>. T\u2010cell hybridomas specific for epitopes from each region were generated to study the mechanisms of processing and presentation of V antigen by bone\u2010marrow\u2010derived macrophages. All three epitopes required uptake and\/or processing from V antigen as well as presentation to T cells by newly synthesized major histocompatibility complex (MHC) class II molecules over a time period of 3\u20134\u2003hr. Sensitivity to inhibitors showed a dependence on low pH and cysteine, serine and metalloproteinase, but not aspartic proteinase, activity. The data indicate that immunodominant epitopes from all three structural regions of V antigen were presented preferentially by the classical MHC class II\u2010restricted presentation pathway. The requirement for processing by the co\u2010ordinated activity of several enzyme families is consistent with the buried location of the epitopes in each region of V antigen. Understanding the structure\u2013function relationship of multiple immunodominant epitopes of candidate subunit vaccines is necessary to inform choice of adjuvants for vaccine delivery. In the case of V antigen, adjuvants designed to target it to lysosomes are likely to induce optimal responses to multiple protective T\u2010cell epitopes.<\/jats:p>","DOI":"10.1111\/j.1365-2567.2006.02447.x","type":"journal-article","created":{"date-parts":[[2006,8,18]],"date-time":"2006-08-18T08:37:55Z","timestamp":1155890275000},"page":"385-392","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Mechanisms of major histocompatibility complex class II\u2010restricted processing and presentation of the V antigen of <i>Yersinia pestis<\/i>"],"prefix":"10.1111","volume":"119","author":[{"given":"Ho\u2010Ki","family":"Shim","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julie A.","family":"Musson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Helen M.","family":"Harper","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hesta V.","family":"McNeill","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicola","family":"Walker","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Helen","family":"Flick\u2010Smith","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexei","family":"Von Delwig","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E. 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