{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T00:13:14Z","timestamp":1792800794169,"version":"4.5.0"},"reference-count":23,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2006,10,9]],"date-time":"2006-10-09T00:00:00Z","timestamp":1160352000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunology"],"published-print":{"date-parts":[[2006,12]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Human papillomaviruses (HPVs) are obligate epithelial pathogens and typically cause localized mucosal infections. We therefore hypothesized that T\u2010cell responses to HPV antigens would be greater at sites of pathology than in the blood. Focusing on HPV\u201016 because of its association with cervical cancer, the magnitude of HPV\u2010specific T\u2010cell responses at the cervix was compared with those in the peripheral blood by intracellular cytokine staining following direct <jats:italic>ex vivo<\/jats:italic> stimulation with both virus\u2010like particles assembled from the major capsid protein L1, and the major HPV oncoprotein, E7. We show that both CD4<jats:sup>+<\/jats:sup> and CD8<jats:sup>+<\/jats:sup> T cells from the cervix responded to the HPV\u201016 antigens and that interferon\u2010\u03b3 (IFN\u2010\u03b3) production was HPV type\u2010specific. Comparing HPV\u2010specific T\u2010cell IFN\u2010\u03b3 responses at the cervix with those in the blood, we found that while CD4<jats:sup>+<\/jats:sup> and CD8<jats:sup>+<\/jats:sup> T\u2010cell responses to L1 were significantly correlated between compartments (<jats:italic>P\u2003=<\/jats:italic>\u20030\u00b702 and <jats:italic>P<\/jats:italic>\u2003=\u20030\u00b705, respectively), IFN\u2010\u03b3 responses in both T\u2010cell subsets were significantly greater in magnitude at the cervix than in peripheral blood (<jats:italic>P\u2003=<\/jats:italic>\u20030\u00b702 and <jats:italic>P<\/jats:italic>\u2003=\u20030\u00b7003, respectively). In contrast, both CD4<jats:sup>+<\/jats:sup> and CD8<jats:sup>+<\/jats:sup> T\u2010cell IFN\u2010\u03b3 responses to E7 were of similar magnitude in both compartments and CD8<jats:sup>+<\/jats:sup> responses were significantly correlated between these distinct immunological compartments (<jats:italic>P\u2003=<\/jats:italic>\u20030\u00b704). We therefore show that inflammatory T\u2010cell responses against L1 (but not E7) demonstrate clear compartmental bias and the magnitude of these responses do reflect local viral replication but that correlation of HPV\u2010specific responses between compartments indicates their linkage.<\/jats:p>","DOI":"10.1111\/j.1365-2567.2006.02465.x","type":"journal-article","created":{"date-parts":[[2006,10,9]],"date-time":"2006-10-09T09:02:49Z","timestamp":1160384569000},"page":"507-514","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["Comparison of cervical and blood T\u2010cell responses to human papillomavirus\u201016 in women with human papillomavirus\u2010associated cervical intraepithelial neoplasia"],"prefix":"10.1111","volume":"119","author":[{"given":"Jo\u2010Ann S.","family":"Passmore","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michelle","family":"Milner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lynnette","family":"Denny","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Candice","family":"Sampson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dianne J.","family":"Marais","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruce","family":"Allan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pam P.","family":"Gumbi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Inga 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