{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,23]],"date-time":"2026-04-23T00:28:13Z","timestamp":1776904093507,"version":"3.51.2"},"reference-count":50,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2006,10,9]],"date-time":"2006-10-09T00:00:00Z","timestamp":1160352000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunology"],"published-print":{"date-parts":[[2006,12]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>In previous studies, we identified a gene product belonging to the silent information regulatory 2 protein (SIR2) family. This protein is expressed by all <jats:italic>Leishmania<\/jats:italic> species so far examined (<jats:italic>L. major<\/jats:italic>, <jats:italic>L. infantum<\/jats:italic>, <jats:italic>L. amazonensis<\/jats:italic>, <jats:italic>L. mexicana<\/jats:italic>) and found to be crucial for parasite survival and virulence. In the present study, we investigated whether a <jats:italic>Leishmania<\/jats:italic> SIR2 recombinant protein (LmSIR2) would affect T\u2010 and B\u2010cell functions in a murine model. <jats:italic>In vitro<\/jats:italic> treatment of spleen cells from normal BALB\/c mice with LmSIR2 showed increased expression of CD69 on B cells. This effect was not abolished by the addition of polymyxin B. Intravenous injection of LmSIR2 into BALB\/c mice induced increased spleen B cell number by a factor of about \u22481\u00b76, whereas no modification occurred at the level of CD4<jats:sup>+<\/jats:sup> and CD8<jats:sup>+<\/jats:sup> cells. Furthermore, intraperitoneal injection of LmSIR2 alone without adjuvant into BALB\/c mice or nude mice triggered the production of elevated levels of LmSIR2\u2010specific antibodies. The analysis of specific isotype profiles showed a predominance of immunoglobulin G1 (IgG1) and IgG2a antibody responses in BALB\/c mice, and IgM in nude mice. Moreover, the anti\u2010LmSIR2 mouse antibodies in the presence of complement induced the <jats:italic>in vitro<\/jats:italic> lysis of <jats:italic>L. infantum<\/jats:italic> amastigotes. In the absence of complement, the antibodies induced significant inhibition of amastigotes developpement inside macrophages. Together, the current study provides the first evidence that a <jats:italic>Leishmania<\/jats:italic> protein belonging to the SIR2 family may play a role in the regulation of immune response through its capacity to trigger B\u2010cell effector function.<\/jats:p>","DOI":"10.1111\/j.1365-2567.2006.02468.x","type":"journal-article","created":{"date-parts":[[2006,10,9]],"date-time":"2006-10-09T09:02:49Z","timestamp":1160384569000},"page":"529-540","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["<i>Leishmania<\/i> cytosolic silent information regulatory protein 2 deacetylase induces murine B\u2010cell differentiation and <i>in vivo<\/i> production of specific 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