{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,9,6]],"date-time":"2024-09-06T21:47:08Z","timestamp":1725659228786},"reference-count":24,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2007,3,26]],"date-time":"2007-03-26T00:00:00Z","timestamp":1174867200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunology"],"published-print":{"date-parts":[[2007,7]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Pemphigus vulgaris (PV) is considered to be an autoimmune disease affecting skin and mucous membranes. Traditionally, PV autoantibodies are thought to recognize antigens located in the intercellular substance (ICS) of keratinocytes; antigens represented mainly by the desmosomal cadherin desmoglein 3 (Dsg3). Accordingly, titres of anti\u2010ICS and anti\u2010Dsg3 immunoglobulin G (IgG) are considered to be major laboratory criteria when making a diagnosis of PV. In this paper, we demonstrated for the first time that PV IgG bind antigen(s) expressed on the surface of peripheral blood mononuclear cells (PBMC), as revealed by immunofluorescence studies. This novel autoantigen is immunoprecipitated by PV IgG as a 130\u2003000 molecular weight protein. However, Western blot analysis of the immunocomplexes failed to show reactivity with anti\u2010Dsg3 monoclonal and polyclonal antibodies. Taken together, our data provide strong evidence that PV autoimmunity targets a 130\u2003000 antigen other than Dsg3 on PBMC. This shifting from epidermis to blood cells may open new perspectives for a better understanding of pemphigus autoimmunity and more rational approaches to its treatment.<\/jats:p>","DOI":"10.1111\/j.1365-2567.2007.02585.x","type":"journal-article","created":{"date-parts":[[2007,5,26]],"date-time":"2007-05-26T16:36:38Z","timestamp":1180197398000},"page":"377-382","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Pemphigus vulgaris immunoglobulin G can recognize a 130\u2003000 MW antigen other than desmoglein 3 on peripheral blood mononuclear cell surface"],"prefix":"10.1111","volume":"121","author":[{"given":"Nicola","family":"Cirillo","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernando","family":"Gombos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alessandro","family":"Lanza","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,3,26]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(05)66829-8"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/229059a0"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(91)90360-B"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.138.1.193"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12471934"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198909073211002"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2003.11.051"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M003174200"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)64651-2"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.0303-6987.2006.00523.x"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1177\/039463200601900216"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2006.07654.x"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2006.04.089"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2249.2004.02570.x"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.106.3.761"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.20856"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.109.1.163"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1601-2006.0825.01287.x"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.7536343"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI5252"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.153.4.823"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI23475"},{"key":"e_1_2_6_24_2","article-title":"Pemphigus acantholysis and steric hindrance: an unsolved equation","author":"Cirillo N","year":"2006","journal-title":"J Clin Invest"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI10305"}],"container-title":["Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1365-2567.2007.02585.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1365-2567.2007.02585.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,13]],"date-time":"2023-10-13T19:53:24Z","timestamp":1697226804000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1365-2567.2007.02585.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,3,26]]},"references-count":24,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2007,7]]}},"alternative-id":["10.1111\/j.1365-2567.2007.02585.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1365-2567.2007.02585.x","archive":["Portico"],"relation":{},"ISSN":["0019-2805","1365-2567"],"issn-type":[{"value":"0019-2805","type":"print"},{"value":"1365-2567","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,3,26]]},"assertion":[{"value":"2007-03-26","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}