{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,1,18]],"date-time":"2024-01-18T18:25:25Z","timestamp":1705602325285},"reference-count":54,"publisher":"Wiley","issue":"1pt2","license":[{"start":{"date-parts":[[2008,12,24]],"date-time":"2008-12-24T00:00:00Z","timestamp":1230076800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunology"],"published-print":{"date-parts":[[2009,9]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Rat major histocompatibility complex (MHC) class II molecules RT1.B<jats:sup>l<\/jats:sup> (DQ\u2010like) and RT1.D<jats:sup>l<\/jats:sup> (DR\u2010like) were cloned from the LEW strain using reverse transcription\u2013polymerase chain reaction and expressed in mouse L929 cells. The transduced lines bound MHC class II\u2010specific monoclonal antibodies in an MHC\u2010isotype\u2010specific manner and presented peptide antigens and superantigens to T\u2010cell hybridomas. The T\u2010cell\u2010hybridomas responded well to all superantigens presented by human MHC class II, whereas the response varied considerably with rat MHC class II\u2010transduced lines as presenters. The T\u2010cell hybridomas responded to the pyrogenic superantigens <jats:italic>Staphylococcus<\/jats:italic> enterotoxin B (SEB), SEC1, SEC2 and SEC3 only at high concentrations with RT1.B<jats:sup>l<\/jats:sup>\u2010transduced and RT1.D<jats:sup>l<\/jats:sup>\u2010transduced cells as presenters. The same was true for streptococcal pyrogenic exotoxin A (SPEA), but this was presented only by RT1.B<jats:sup>l<\/jats:sup> and not by RT1.D<jats:sup>l<\/jats:sup>. SPEC was recognized only if presented by human MHC class II. Presentation of <jats:italic>Yersinia pseudotuberculosis<\/jats:italic> superantigen (YPM) showed no MHC isotype preference, while <jats:italic>Mycoplasma arthritidis<\/jats:italic> superantigen (MAS or MAM) was presented by RT1.D<jats:sup>l<\/jats:sup> but not by RT1.B<jats:sup>l<\/jats:sup>. Interestingly, and in contrast to RT1.B<jats:sup>l<\/jats:sup>, the RT1.D<jats:sup>l<\/jats:sup> completely failed to present SEA and toxic shock syndrome toxin 1 even after transduction of invariant chain (CD74) or expression in other cell types such as the surface MHC class II\u2010negative mouse B\u2010cell lymphoma (M12.4.1.C3). We discuss the idea that a lack of SEA presentation may not be a general feature of RT1.D molecules but could be a consequence of RT1.D<jats:sup>l<\/jats:sup>\u03b2\u2010chain allele\u2010specific substitutions (arginine 80 to lysine, asparagine 82 to aspartic acid) in the extremely conserved region flanking the Zn<jats:sup>2+<\/jats:sup>\u2010binding histidine 81, which is crucial for high\u2010affinity SEA\u2010binding.<\/jats:p>","DOI":"10.1111\/j.1365-2567.2008.03033.x","type":"journal-article","created":{"date-parts":[[2008,12,24]],"date-time":"2008-12-24T11:36:07Z","timestamp":1230118567000},"update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Superantigen\u2010presentation by rat major histocompatibility complex class II molecules RT1.B<sup>l<\/sup> and RT1.D<sup>l<\/sup>"],"prefix":"10.1111","volume":"128","author":[{"given":"Henry","family":"Dlaske","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hatice","family":"Kara\u00fcz\u00fcm","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elisa","family":"Monzon\u2010Casanova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ronald","family":"Rudolf","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lisa","family":"Starick","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ingrid","family":"M\u00fcller","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gerhild","family":"Wildner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria","family":"Diedrichs\u2010M\u00f6hring","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Norbert","family":"Koch","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tohru","family":"Miyoshi\u2010Akiyama","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takehiko","family":"Uchiyama","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kurt","family":"Wonigeit","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bernhard","family":"Fleischer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silke","family":"Overbeck","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lothar","family":"Rink","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Herrmann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,8,4]]},"reference":[{"key":"e_1_2_8_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.smim.2007.04.006"},{"key":"e_1_2_8_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0952-7915(01)00296-5"},{"key":"e_1_2_8_4_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2249.2003.02203.x"},{"key":"e_1_2_8_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/dxh068"},{"key":"e_1_2_8_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830220738"},{"key":"e_1_2_8_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0923-2494(92)80122-2"},{"key":"e_1_2_8_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780350413"},{"key":"e_1_2_8_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.2008.00681.x"},{"key":"e_1_2_8_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780360717"},{"key":"e_1_2_8_11_2","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1016\/S1438-4221(00)80072-7","article-title":"The 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