{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,13]],"date-time":"2026-11-13T12:05:15Z","timestamp":1794571515893,"version":"4.9.1"},"reference-count":59,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2010,1,27]],"date-time":"2010-01-27T00:00:00Z","timestamp":1264550400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunology"],"published-print":{"date-parts":[[2010,6]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    Dengue fever (DF), a public health problem in tropical countries, may present severe clinical manifestations as result of increased vascular permeability and coagulation disorders. Dengue virus (DENV), detected in peripheral monocytes during acute disease and in\n                    <jats:italic>in vitro<\/jats:italic>\n                    infection, leads to cytokine production, indicating that virus\u2013target cell interactions are relevant to pathogenesis. Here we investigated the\n                    <jats:italic>in vitro<\/jats:italic>\n                    and\n                    <jats:italic>in vivo<\/jats:italic>\n                    activation of human peripheral monocytes after DENV infection. The numbers of CD14\n                    <jats:sup>+<\/jats:sup>\n                    monocytes expressing the adhesion molecule intercellular adhesion molecule 1 (ICAM\u20101) were significantly increased during acute DF. A reduced number of CD14\n                    <jats:sup>+<\/jats:sup>\n                    \u2003human leucocyte antigen (HLA)\u2010DR\n                    <jats:sup>+<\/jats:sup>\n                    monocytes was observed in patients with severe dengue when compared to those with mild dengue and controls; CD14\n                    <jats:sup>+<\/jats:sup>\n                    monocytes expressing toll\u2010like receptor (TLR)2 and TLR4 were increased in peripheral blood from dengue patients with mild disease, but\n                    <jats:italic>in vitro<\/jats:italic>\n                    DENV\u20102 infection up\u2010regulated only TLR2. Increased numbers of CD14\n                    <jats:sup>+<\/jats:sup>\n                    \u2003CD16\n                    <jats:sup>+<\/jats:sup>\n                    activated monocytes were found after\n                    <jats:italic>in vitro<\/jats:italic>\n                    and\n                    <jats:italic>in vivo<\/jats:italic>\n                    DENV\u20102 infection. The CD14\n                    <jats:sup>high<\/jats:sup>\n                    \u2003CD16\n                    <jats:sup>+<\/jats:sup>\n                    monocyte subset was significantly expanded in mild dengue, but not in severe dengue. Increased plasma levels of tumour necrosis factor\u2010\u03b1 (TNF\u2010\u03b1), interferon\u2010\u03b3 (IFN\u2010\u03b3) and interleukin (IL)\u201018 in dengue patients were inversely associated with CD14\n                    <jats:sup>high<\/jats:sup>\n                    \u2003CD16\n                    <jats:sup>+<\/jats:sup>\n                    , indicating that these cells might be involved in controlling exacerbated inflammatory responses, probably by IL\u201010 production. We showed here, for the first time, phenotypic changes on peripheral monocytes that were characteristic of cell activation. A sequential monocyte\u2010activation model is proposed in which DENV infection triggers TLR2\/4 expression and inflammatory cytokine production, leading eventually to haemorrhagic manifestations, thrombocytopenia, coagulation disorders, plasmatic leakage and shock development, but may also produce factors that act in order to control both intense immunoactivation and virus replication.\n                  <\/jats:p>","DOI":"10.1111\/j.1365-2567.2009.03224.x","type":"journal-article","created":{"date-parts":[[2010,1,27]],"date-time":"2010-01-27T05:14:39Z","timestamp":1264569279000},"page":"202-216","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":98,"title":["Differential regulation of toll\u2010like receptor\u20102, toll\u2010like receptor\u20104, CD16 and human leucocyte antigen\u2010DR on peripheral blood monocytes during mild and severe dengue fever"],"prefix":"10.1111","volume":"130","author":[{"given":"Elzinandes L.","family":"Azeredo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patr\u00edcia C.","family":"Neves\u2010Souza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Allan R.","family":"Alvarenga","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00f4nia R. N. I.","family":"Reis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amanda","family":"Torrentes\u2010Carvalho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sonia\u2010Maris O.","family":"Zagne","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rita M. 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