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To achieve this broad cellular immune response we tested a heterologous DNA\/protein combination vaccine strategy. We used a purified recombinant protein preparation of a unique <jats:italic>M.\u00a0tuberculosis<\/jats:italic> antigen (rMT1721) found in the urine of TB patients, an optimized plasmid DNA expressing this protein (DNA\u2010MT1721), and a Toll\u2010like receptor 4 agonist adjuvant. We found that priming mice with DNA\u2010MT1721 and subsequently boosting with rMT1721 elicited high titres of specific IgG1 and IgG2a antibodies as well as high magnitude and polyfunctional CD4<jats:sup>+<\/jats:sup> T\u2010cell responses. However, no detectable CD8<jats:sup>+<\/jats:sup> T\u2010cell response was observed using this regimen of immunization. In contrast, both CD4<jats:sup>+<\/jats:sup> and CD8<jats:sup>+<\/jats:sup> T\u2010cell responses were detected after a prime\/boost vaccination regimen using rMT1721 as the priming antigen and DNA\u2010MT1721 as the boosting immunogen. These findings support the exploration of heterologous DNA\/protein immunization strategies in vaccine development against TB and possibly other infectious diseases.<\/jats:p>","DOI":"10.1111\/j.1365-2567.2011.03525.x","type":"journal-article","created":{"date-parts":[[2011,10,21]],"date-time":"2011-10-21T19:09:35Z","timestamp":1319224175000},"page":"216-225","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Robust immune response elicited by a novel and unique <i>Mycobacterium tuberculosis<\/i> protein using an optimized DNA\/protein heterologous prime\/boost protocol"],"prefix":"10.1111","volume":"135","author":[{"given":"Mark J.","family":"Cayabyab","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suely 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