{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,15]],"date-time":"2023-10-15T04:16:15Z","timestamp":1697343375065},"reference-count":32,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2012,1,24]],"date-time":"2012-01-24T00:00:00Z","timestamp":1327363200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunology"],"published-print":{"date-parts":[[2012,3]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>We have previously reported that the 20\u2010mer peptide p2340 (amino acids 2340\u20132359), of human thyroglobulin (Tg) has the unique feature that it causes experimental autoimmune thyroiditis (EAT) in mouse strains bearing high\u2010responder (HR) or low\u2010responder (LR) MHC haplotypes in Tg\u2010induced EAT. In this study, we have employed fine epitope mapping to examine whether this property of p2340 is the result of recognition of distinct or shared minimal T\u2010cell epitopes in the context of HR or LR MHC class II molecules. Use of overlapping peptides showed that a core minimal 9\u2010mer epitope (LTWVQTHIR, amino acids 2344\u20132352) was recognized by p2340\u2010primed T cells from both HR (<jats:italic>H2<\/jats:italic><jats:sup>k,s<\/jats:sup>) and LR (<jats:italic>H2<\/jats:italic><jats:sup>b,d<\/jats:sup>) strains, whereas a second 9\u2010mer epitope (HIRGFGGDP, amino acids 2350\u20132358) was antigenic only in <jats:italic>H2<\/jats:italic><jats:sup>s<\/jats:sup> hosts. Truncation analysis of LTWVQTHIR and HIRGFGGDP peptides delineated them as the minimal epitopes recognized by p2340\u2010primed T cells from the above strains. Subcutaneous challenge of all mouse strains with the 9\u2010mer core peptide LTWVQTHIR in adjuvant elicited specific lymph node cell proliferative responses and mild EAT only in HR hosts, highlighting this sequence as a minimal pathogenic Tg peptide in EAT. The 9\u2010mer peptide HIRGFGGDP was not found to be immunogenic in <jats:italic>H2<\/jats:italic><jats:sup>s<\/jats:sup> hosts. These data demonstrate that minimal T\u2010cell epitopes, defined as autoantigenic in hosts of various MHC haplotypes, are not intrinsically immunogenic. Activation of naive autoreactive T cells may require contributions from flanking residues within longer peptide sequences encompassing these epitopes.<\/jats:p>","DOI":"10.1111\/j.1365-2567.2011.03538.x","type":"journal-article","created":{"date-parts":[[2011,11,21]],"date-time":"2011-11-21T02:08:43Z","timestamp":1321841323000},"page":"245-253","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Fine epitope mapping within the pathogenic thyroglobulin peptide 2340\u20132359: minimal epitopes retaining antigenicity across various MHC haplotypes are not necessarily immunogenic"],"prefix":"10.1111","volume":"135","author":[{"given":"Aikaterini","family":"Hatzioannou","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria","family":"Alevizaki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"George","family":"Carayanniotis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peggy","family":"Lymberi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,1,24]]},"reference":[{"key":"e_1_2_8_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/0471142735.im1507s78"},{"key":"e_1_2_8_3_2","first-page":"245","article-title":"Murine thyroiditis III. 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