{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T10:43:34Z","timestamp":1762080214094,"version":"build-2065373602"},"reference-count":30,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,1,21]],"date-time":"2005-01-21T00:00:00Z","timestamp":1106265600000},"content-version":"vor","delay-in-days":20,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["resjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Insect Molecular Biology"],"published-print":{"date-parts":[[2005,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Germline transformation using a\n                    <jats:italic>piggyBac<\/jats:italic>\n                    \u2010derived vector is feasible in the sawfly,\n                    <jats:italic>Athalia rosae<\/jats:italic>\n                    . A previously generated transgenic line carrying green fluorescence protein (GFP) genes as reporters was successfully maintained and preserved without consecutive rearing. Sperm taken from males that were frozen directly in liquid nitrogen and stored at \u221280\u00a0\u00b0C for a year were microinjected into mature unfertilized eggs dissected from female ovaries. A fraction of the sperm\u2010injected eggs was fertilized and developed into diploid females, and all of them expressed GFP. Haploid male progeny from these females segregated into GFP\u2010positive and GFP\u2010negative individuals in a ratio of 1\u00a0:\u00a01 indicating heterozygosity of the parental females. The GFP genes were stably inherited staying at the location where they were originally integrated.\n                  <\/jats:p>","DOI":"10.1111\/j.1365-2583.2004.00526.x","type":"journal-article","created":{"date-parts":[[2005,1,21]],"date-time":"2005-01-21T11:32:15Z","timestamp":1106307135000},"page":"105-109","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Preservation of a transgenic strain of the sawfly,\n                    <i>Athalia rosae<\/i>\n                    (Hymenoptera) by artificial fertilization using cryopreserved sperm"],"prefix":"10.1111","volume":"14","author":[{"given":"M.","family":"Hatakeyama","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Sumitani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,1,21]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0965-1748(02)00087-5"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0965-1748(02)00086-3"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ento.46.1.317"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(89)90117-7"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1201\/9781420039399.ch14"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0965-1748(02)00084-X"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2003.08.005"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1520-6327(200003)43:3<137::AID-ARCH5>3.0.CO;2-E"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1080\/07924259.1990.9672116"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1910(94)90025-6"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.140142697"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt769"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1020958831972"},{"key":"e_1_2_5_15_1","first-page":"37","article-title":"Cryopreservation of sheep blow fly embryos, Lucilia cuprina (Diptera: Calliphoridae)","volume":"20","author":"Leopold R.A.","year":"1999","journal-title":"Cryo-Letters"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1603\/0013-8746(2001)094[0695:COEOTN]2.0.CO;2"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/4.1.43"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1470915"},{"key":"e_1_2_5_19_1","first-page":"569","article-title":"Chromosome number differentiation in sawflies and its systematic implication (Hymenoptera, Tenthredinidae)","volume":"50","author":"Naito T.","year":"1982","journal-title":"Kontyu (Tokyo)"},{"key":"e_1_2_5_20_1","first-page":"1","article-title":"Developmental biology of the sawfly, Athalia rosae (Hymenoptera)","volume":"30","author":"Oishi K.","year":"1995","journal-title":"Proc Arthropod Embryol Soc Jpn"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02424468"},{"key":"e_1_2_5_22_1","first-page":"125","article-title":"Cryopreservation of Mediterranean fruit fly embryos","volume":"24","author":"Rajamohan A.","year":"2003","journal-title":"Cryo-Letters"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2003.10.004"},{"key":"e_1_2_5_24_1","first-page":"541","article-title":"Studies on the sawfly, Athalia rosae (Insecta, Hymenoptera, Tenthredinidae). 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