{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T11:37:15Z","timestamp":1790854635130,"version":"4.1.0"},"reference-count":64,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2005,7,15]],"date-time":"2005-07-15T00:00:00Z","timestamp":1121385600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["resjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Insect Molecular Biology"],"published-print":{"date-parts":[[2005,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Ixodes scapularis<\/jats:italic>\n                    transmits several pathogens including\n                    <jats:italic>Borrelia burgdorferi<\/jats:italic>\n                    . Bioactive compounds in tick saliva support tick feeding and influence pathogen transmission to the mammalian host. These studies utilized oral delivery of dsRNA to silence an anticomplement gene (\n                    <jats:italic>isac<\/jats:italic>\n                    ) in\n                    <jats:italic>I.\u00a0scapularis<\/jats:italic>\n                    nymphs. Silencing of\n                    <jats:italic>isac<\/jats:italic>\n                    significantly reduced fed\u2010tick weight compared to delivery of control\n                    <jats:italic>lacZ<\/jats:italic>\n                    dsRNA, and immunoblots specific for FlaB protein indicated a reduction in spirochete load in\n                    <jats:italic>isac<\/jats:italic>\n                    \u2010silenced infected nymphs. SDS\u2010PAGE demonstrated that\n                    <jats:italic>isac<\/jats:italic>\n                    gene silencing affected expression of a number of salivary and non\u2010salivary gland proteins in ticks. Finally, multiple\n                    <jats:italic>isac<\/jats:italic>\n                    cDNA homologues were cloned, and these may represent a new gene family coexpressed during tick feeding. This work presents a novel oral delivery approach for specific gene silencing in\n                    <jats:italic>I.\u00a0scapularis<\/jats:italic>\n                    nymphs and characterizes the effect of\n                    <jats:italic>isac<\/jats:italic>\n                    on blood\u2010feeding in an attempt to block transmission of\n                    <jats:italic>B.\u00a0burgdorferi<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1111\/j.1365-2583.2005.00575.x","type":"journal-article","created":{"date-parts":[[2005,7,19]],"date-time":"2005-07-19T10:53:44Z","timestamp":1121770424000},"page":"443-452","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":70,"title":["Capillary feeding of specific dsRNA induces silencing of the\n                    <i>isac<\/i>\n                    gene in nymphal\n                    <i>Ixodes scapularis<\/i>\n                    ticks"],"prefix":"10.1111","volume":"14","author":[{"given":"C. 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