{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T16:23:05Z","timestamp":1762100585745,"version":"build-2065373602"},"reference-count":16,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2006,10,27]],"date-time":"2006-10-27T00:00:00Z","timestamp":1161907200000},"content-version":"vor","delay-in-days":26,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["resjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Insect Molecular Biology"],"published-print":{"date-parts":[[2006,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Several components of honeybee venom are known to cause allergenic responses in humans and other vertebrates. One such component, the minor allergen Api m 6, has been known to show amino acid variation but the genetic mechanism for this variation is unknown. Here we show that Api m 6 is derived from a single locus, and that substantial protein\u2010level variation has a simple genome\u2010level cause, without the need to invoke multiple loci or alternatively spliced exons. Api m 6 sits near a misassembled section of the honeybee genome sequence, and we propose that a substantial number of indels at and near Api m 6 might be the root cause of this misassembly. We suggest that genes such as Api m 6 with coding\u2010region or untranslated region indels might have had a strong effect on the assembly of this draft of the honeybee genome.<\/jats:p>","DOI":"10.1111\/j.1365-2583.2006.00669.x","type":"journal-article","created":{"date-parts":[[2006,10,27]],"date-time":"2006-10-27T09:24:19Z","timestamp":1161941059000},"page":"577-581","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Genomic and transcriptional analysis of protein heterogeneity of the honeybee venom allergen Api m 6"],"prefix":"10.1111","volume":"15","author":[{"given":"N.","family":"Peiren","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. C.","family":"De Graaf","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. D.","family":"Evans","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F. J.","family":"Jacobs","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,10,27]]},"reference":[{"key":"e_1_2_4_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.17.3389"},{"key":"e_1_2_4_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-554770-3.50011-5"},{"key":"e_1_2_4_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2004.05.028"},{"key":"e_1_2_4_5_1","doi-asserted-by":"crossref","first-page":"26107","DOI":"10.1016\/S0021-9258(18)47165-7","article-title":"Biodiversity of apidaecin\u2010type peptide antibiotics \u2013 prospects of manipulating the antibacterial spectrum and combating acquired\u2010resistance","volume":"269","author":"Casteels P.","year":"1994","journal-title":"J Biol Chem"},{"key":"e_1_2_4_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00122-004-1856-9"},{"key":"e_1_2_4_7_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.21.12704"},{"key":"e_1_2_4_8_1","first-page":"123","article-title":"Fatal reactions to hymenoptera stings","volume":"24","author":"Hoffman D.R.","year":"2003","journal-title":"Allergy Asthma Proc"},{"key":"e_1_2_4_9_1","doi-asserted-by":"publisher","DOI":"10.1385\/CRIAI:30:2:109"},{"key":"e_1_2_4_10_1","doi-asserted-by":"publisher","DOI":"10.1067\/mai.2001.113867"},{"key":"e_1_2_4_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1980.tb06073.x"},{"key":"e_1_2_4_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-6749(96)70301-9"},{"key":"e_1_2_4_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkh454"},{"key":"e_1_2_4_14_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1005352410725"},{"key":"e_1_2_4_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/mnfr.200500020"},{"key":"e_1_2_4_16_1","first-page":"365","volume-title":"Bioinformatics, Methods, and Protocols: Methods in Molecular, Biology","author":"Rozen S.","year":"2000"},{"key":"e_1_2_4_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(73)80226-1"}],"container-title":["Insect Molecular Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1365-2583.2006.00669.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/resjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1365-2583.2006.00669.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T07:55:14Z","timestamp":1762070114000},"score":1,"resource":{"primary":{"URL":"https:\/\/resjournals.onlinelibrary.wiley.com\/doi\/10.1111\/j.1365-2583.2006.00669.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,10]]},"references-count":16,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2006,10]]}},"alternative-id":["10.1111\/j.1365-2583.2006.00669.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1365-2583.2006.00669.x","archive":["Portico"],"relation":{},"ISSN":["0962-1075","1365-2583"],"issn-type":[{"type":"print","value":"0962-1075"},{"type":"electronic","value":"1365-2583"}],"subject":[],"published":{"date-parts":[[2006,10]]},"assertion":[{"value":"2006-10-27","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}