{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T07:20:58Z","timestamp":1793344858148,"version":"4.5.0"},"reference-count":21,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2006,12,19]],"date-time":"2006-12-19T00:00:00Z","timestamp":1166486400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["resjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Insect Molecular Biology"],"published-print":{"date-parts":[[2007,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Sex\u2010specific expression of transgenes in pest insects enables novel genetic control strategies, based either on genetic sexing or the spread of transgenes through the germ\u2010line, to be developed and then tested for implementation. We describe the isolation of the \u03b2 tubulin genes from the yellow fever mosquito,\n                    <jats:italic>Aedes aegypti<\/jats:italic>\n                    , and the identification of the particular \u03b22 tubulin gene which has expression confined to the testes. We demonstrate that the \u03b22 tubulin promoter of\n                    <jats:italic>Ae.\u00a0aegypti<\/jats:italic>\n                    can direct the expression of a DsRed genetic marker in the testes and show that labelled sperm can be detected in inseminated spermathecae. The applications for this technology in the genetic control of\n                    <jats:italic>Ae.\u00a0aegypti<\/jats:italic>\n                    are discussed.\n                  <\/jats:p>","DOI":"10.1111\/j.1365-2583.2006.00701.x","type":"journal-article","created":{"date-parts":[[2006,12,20]],"date-time":"2006-12-20T21:00:03Z","timestamp":1166648403000},"page":"61-71","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":101,"title":["Testis\u2010specific expression of the \u03b22 tubulin promoter of\n                    <i>Aedes aegypti<\/i>\n                    and its application as a genetic sex\u2010separation marker"],"prefix":"10.1111","volume":"16","author":[{"given":"R. C.","family":"Smith","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. F.","family":"Walter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. H.","family":"Hice","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. A.","family":"O\u2019Brochta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. W.","family":"Atkinson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,12,19]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1471-4922(03)00144-2"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1152"},{"key":"e_1_2_6_4_1","volume-title":"Aedes aegypti (L.). The Yellow Fever Mosquito. Its Life History, Bionomics and Structure","author":"Christophers S.R.","year":"1960"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/1-4020-4051-2_16"},{"key":"e_1_2_6_6_1","first-page":"355","article-title":"Female polygamy due to inadequate semen transfer in Aedes aegypti","volume":"30","author":"Gwadz R.W.","year":"1970","journal-title":"Mosquito News"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/jee\/46.4.624"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvg.1020160208"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/jmedent\/33.6.955"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0965-1748(02)00184-4"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(82)90321-X"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0012-1606(89)80014-4"},{"key":"e_1_2_6_13_1","first-page":"66","article-title":"An 18\u2010bp element in the 5\u2032 untranslated region of the Drosophila\u03b22 tubulin mRNA regulates the mRNA level postmeiotic stages of spermatogenesis","volume":"62","author":"Michiels F.","year":"1993","journal-title":"Eur J Cell Bio"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1989.tb03540.x"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00333989"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-94-009-1535-0_35"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-94-009-1535-0_2"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/25126"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.7.6.2231"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/28.6.1439"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00427-004-0400-x"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/jmedent\/23.4.374"}],"container-title":["Insect Molecular Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1365-2583.2006.00701.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/resjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1365-2583.2006.00701.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T11:00:45Z","timestamp":1762081245000},"score":1,"resource":{"primary":{"URL":"https:\/\/resjournals.onlinelibrary.wiley.com\/doi\/10.1111\/j.1365-2583.2006.00701.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,12,19]]},"references-count":21,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2007,2]]}},"alternative-id":["10.1111\/j.1365-2583.2006.00701.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1365-2583.2006.00701.x","archive":["Portico"],"relation":{},"ISSN":["0962-1075","1365-2583"],"issn-type":[{"value":"0962-1075","type":"print"},{"value":"1365-2583","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,12,19]]},"assertion":[{"value":"2006-12-19","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}