{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T09:50:00Z","timestamp":1768902600333,"version":"3.49.0"},"reference-count":40,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2007,4,13]],"date-time":"2007-04-13T00:00:00Z","timestamp":1176422400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["resjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Insect Molecular Biology"],"published-print":{"date-parts":[[2007,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Two genes encoding sterol carrier protein\u20102 like proteins are identified from fourth instar cDNAs of the yellow fever mosquito,\n                    <jats:italic>Aedes aegypti<\/jats:italic>\n                    . The predicted AeSCP\u20102like1 (AeSCP\u20102L1) and AeSCP\u20102like2 (AeSCP\u20102L2) proteins are small, acidic and lacking the peroxisomal targeting sequence at the C\u2010termini. Purified recombinant AeSCP\u20102L1 and \u20102L2 bind to cholesterol with a\n                    <jats:italic>K<\/jats:italic>\n                    <jats:sub>d<\/jats:sub>\n                    of 5.4\u00a0\u00d7\u00a010\n                    <jats:sup>\u22126<\/jats:sup>\n                    \u00a0M and 2.6\u00a0\u00d7\u00a010\n                    <jats:sup>\u22126<\/jats:sup>\n                    \u00a0M, respectively. The\n                    <jats:italic>K<\/jats:italic>\n                    <jats:sub>d<\/jats:sub>\n                    values of AeSCP\u20102L1 and \u20102L2 to palmitic acid are 3.7\u00a0\u00d7\u00a010\n                    <jats:sup>\u22127<\/jats:sup>\n                    \u00a0M and 2.6\u00a0\u00d7\u00a010\n                    <jats:sup>\u22127<\/jats:sup>\n                    \u00a0M, respectively. Both genes are expressed predominantly in gut tissues. The transcripts of the\n                    <jats:italic>AeSCP\u20102L1<\/jats:italic>\n                    gene are only detected in larval stages, whereas\n                    <jats:italic>AeSCP\u20102L2<\/jats:italic>\n                    is expressed in larval and adult stages.\n                    <jats:italic>AeSCP\u20102L2<\/jats:italic>\n                    transcription increases within 5\u00a0h after a bloodmeal and stays at high levels during vitellogenesis. In\n                    <jats:italic>in vitro<\/jats:italic>\n                    larval gut tissue cultures,\n                    <jats:italic>AeSCP\u20102L1<\/jats:italic>\n                    transcripts were increased in the presence of juvenile hormone III, whereas\n                    <jats:italic>AeSCP\u20102L2<\/jats:italic>\n                    mRNA levels increased in the presence 20\u2010hydroxylecdysone. The results suggest that transcription of\n                    <jats:italic>AeSCP\u20102L1<\/jats:italic>\n                    and\n                    <jats:italic>\u20102L2<\/jats:italic>\n                    genes are regulated differently through the mosquito life cycle.\n                  <\/jats:p>","DOI":"10.1111\/j.1365-2583.2007.00729.x","type":"journal-article","created":{"date-parts":[[2007,4,13]],"date-time":"2007-04-13T07:56:07Z","timestamp":1176450967000},"page":"305-314","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Identification of two sterol carrier protein\u20102 like genes in the yellow fever mosquito,\n                    <i>Aedes aegypti<\/i>"],"prefix":"10.1111","volume":"16","author":[{"given":"I.","family":"Vyazunova","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V.","family":"Wessley","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Q.","family":"Lan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,4,13]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0965-1748(00)00102-8"},{"key":"e_1_2_6_3_1","volume-title":"Current Protocols in Molecular Biology","author":"Ausubel F.M.","year":"1999"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/arch.940050208"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2583.2005.00560.x"},{"key":"e_1_2_6_6_1","doi-asserted-by":"crossref","first-page":"1042","DOI":"10.1016\/S0022-2275(20)40661-3","article-title":"Diacylglycerol\u2010carrying lipoprotein of hemolymph of the locust and some insects","volume":"22","author":"Chino H.","year":"1981","journal-title":"J Lipid Res"},{"key":"e_1_2_6_7_1","volume-title":"Aedes aegypti (L.). The Yellow Fever Mosquito. Its Life History, Bionomics and Structure","author":"Christophers S.R.","year":"1960"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)46930-X"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M306214200"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00429-003-0324-7"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.50.31878"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.27.16075"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0009-3084(99)00128-0"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(83)90429-3"},{"key":"e_1_2_6_15_1","volume-title":"Introduction to Biological Membranes","author":"Jain M.K.","year":"1988"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/arch.10017"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/0300-9629(94)90039-6"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2583.2003.00386.x"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/arch.940070403"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinsphys.2004.04.012"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.M400003-JLR200"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0962-1075.2004.00510.x"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1910(87)90011-4"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4644(19990401)73:1<70::AID-JCB8>3.0.CO;2-K"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2760(96)00044-6"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0005-2760(97)00003-9"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1603\/0022-2585-40.4.498"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00033a042"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M101939200"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.32.18723"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00919715"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1949.tb27297.x"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M000431200"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1177\/153537020122601002"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1388-1981(00)00047-0"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.50.35425"},{"key":"e_1_2_6_37_1","first-page":"305","article-title":"Intracellular cholesterol trafficking","volume":"14","author":"Sviridov D.","year":"1999","journal-title":"Histol Histopathol"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02535107"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/22.22.4673"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mod.2006.05.005"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1077061"}],"container-title":["Insect Molecular Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1365-2583.2007.00729.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/resjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1365-2583.2007.00729.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T07:54:51Z","timestamp":1762070091000},"score":1,"resource":{"primary":{"URL":"https:\/\/resjournals.onlinelibrary.wiley.com\/doi\/10.1111\/j.1365-2583.2007.00729.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,4,13]]},"references-count":40,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2007,6]]}},"alternative-id":["10.1111\/j.1365-2583.2007.00729.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1365-2583.2007.00729.x","archive":["Portico"],"relation":{},"ISSN":["0962-1075","1365-2583"],"issn-type":[{"value":"0962-1075","type":"print"},{"value":"1365-2583","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,4,13]]},"assertion":[{"value":"2007-04-13","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}