{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T18:05:50Z","timestamp":1793210750064,"version":"4.5.0"},"reference-count":36,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2011,12,6]],"date-time":"2011-12-06T00:00:00Z","timestamp":1323129600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["resjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Insect Molecular Biology"],"published-print":{"date-parts":[[2012,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Aedes aegypti<\/jats:italic>\n                    is an important vector of the viruses that cause dengue fever, dengue haemorrhagic fever and yellow fever. Reverse genetic approaches to the study of gene function in this mosquito have been limited by the lack of a robust inducible promoter to allow precise temporal control over a protein\u2010encoding or hairpin RNA transgene. Likewise, investigations into the molecular and biochemical basis of vector competence would benefit from the ability to activate an antipathogen molecule at specific times during infection. We have characterized the ability of genomic sequences derived from two\n                    <jats:italic>Ae.\u2003aegypti heat shock protein 70<\/jats:italic>\n                    (\n                    <jats:italic>hsp70<\/jats:italic>\n                    ) genes to drive heat\u2010inducible expression of a reporter in both transient and germline transformation contexts.\n                    <jats:italic>AaHsp70<\/jats:italic>\n                    \u2010luciferase transcripts accumulated specifically after heat shock, and displayed a pattern of rapid induction and decay similar to endogenous\n                    <jats:italic>AaHsp70<\/jats:italic>\n                    genes. Luciferase expression in transgenic\n                    <jats:italic>Ae.\u2003aegypti<\/jats:italic>\n                    increased by \u223c25\u201350\u2010fold in whole adults by 4\u2003h after heat\u2010shock, with significant activity (\u223c20\u2010fold) remaining at 24\u2003h. Heat\u2010induced expression was even more dramatic in midgut tissues, with one strain showing a \u223c2500\u2010fold increase in luciferase activity. The\n                    <jats:italic>AaHsp70<\/jats:italic>\n                    promoters described could be valuable for gene function studies as well as for the precise timing of the expression of antipathogen molecules.\n                  <\/jats:p>","DOI":"10.1111\/j.1365-2583.2011.01116.x","type":"journal-article","created":{"date-parts":[[2011,12,6]],"date-time":"2011-12-06T01:42:32Z","timestamp":1323135752000},"page":"97-106","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Robust heat\u2010inducible gene expression by two endogenous\n                    <i>hsp70<\/i>\n                    \u2010derived promoters in transgenic\n                    <i>Aedes aegypti<\/i>"],"prefix":"10.1111","volume":"21","author":[{"given":"T. L. 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