{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T15:17:17Z","timestamp":1759331837632},"reference-count":21,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2005,6,7]],"date-time":"2005-06-07T00:00:00Z","timestamp":1118102400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int Endodontic J"],"published-print":{"date-parts":[[2005,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>Aim\u2002<\/jats:bold> To investigate the occurrence of apoptotic cell death in the epithelium of radicular cysts and to compare its frequency in lesions presenting a distinct functional state.<\/jats:p><jats:p><jats:bold>Methodology\u2002<\/jats:bold> Twenty radicular cysts were selected and arranged into two groups with 10 lesions in each group: atrophic (quiescent) and hyperplastic (active) epithelium. Morphologic investigations of apoptosis were conducted by means of optic microscopy in haematoxylin and eosin slides. Immunohistochemical techniques to detect the bcl\u20102 protein were carried out by streptavidin\u2013biotin\u2013peroxidase assay. In both instances, 30 sequential high\u2010power microscopic fields were observed to determine apoptotic (AI) and bcl\u20102 immunostaining (bcl\u20102I) indexes. The presence of AI and bcl\u20102I within the two groups was compared using the <jats:italic>t<\/jats:italic>\u2010test. Correlation between the AI and the bcl\u20102I was investigated using the Spearman test.<\/jats:p><jats:p><jats:bold>Results\u2002<\/jats:bold> Apoptosis was detected in the epithelium of all cysts. Higher AI levels were found in lesions with an atrophic (0.17\u2003\u00b1\u20030.19) rather than a hyperplastic (0.10\u2003\u00b1\u20030.10) epithelium. The same was found for the bcl\u20102I levels (0.06\u2003\u00b1\u20030.03 vs. 0.04\u2003\u00b1\u20030.01, respectively). However, these differences were not statistically significant. A positive and significant correlation was found between AI and bcl\u20102I.<\/jats:p><jats:p><jats:bold>Conclusions\u2002<\/jats:bold> Apoptosis was always present in the epithelium of the lesions and was more frequent in lesions with atrophic (quiescent) epithelium.<\/jats:p>","DOI":"10.1111\/j.1365-2591.2005.00971.x","type":"journal-article","created":{"date-parts":[[2007,3,21]],"date-time":"2007-03-21T02:05:21Z","timestamp":1174442721000},"page":"465-469","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Apoptosis in epithelial cells of apical radicular cysts"],"prefix":"10.1111","volume":"38","author":[{"given":"A. M.","family":"Loyola","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. V.","family":"Cardoso","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G. S.","family":"Lisa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L. J.","family":"Oliveira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. A.","family":"Mesquita","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. A. V.","family":"Carmo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. C. F.","family":"Aguiar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,6,7]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.281.5381.1322"},{"key":"e_1_2_7_3_1","volume-title":"BioEstat 3.0: aplica\u00e7\u00f5es estat\u00edsticas nas \u00e1reas das ci\u00eancias biol\u00f3gicas e m\u00e9dicas","author":"Ayres M","year":"2003"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1044-579X(02)00127-X"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1014858707218"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0099-2399(98)80210-0"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1177\/154405910208100114"},{"key":"e_1_2_7_8_1","first-page":"1","article-title":"Assessing proliferation and apoptosis in liver tumor development","volume":"15","author":"Goldsworthy TL","year":"1995","journal-title":"CIIT Activities"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-9969(98)00094-6"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0714.2003.00052.x"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9969(96)00032-5"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2591.2000.00276.x"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1067\/moe.2002.123496"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2559.2002.1340f.x"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/bgg044"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2591.2003.00657.x"},{"key":"e_1_2_7_17_1","first-page":"229","article-title":"Necrosis and apoptosis: distinct modes of cell death with fundamentally different significance","volume":"17","author":"Searle J","year":"1982","journal-title":"Pathology Annual"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0714.1985.tb00465.x"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0714.1975.tb01749.x"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1601-0825.1999.tb00096.x"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0300-9785(83)80075-1"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-8986.1989.tb03159.x"}],"container-title":["International Endodontic Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1365-2591.2005.00971.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1365-2591.2005.00971.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,13]],"date-time":"2023-10-13T04:21:07Z","timestamp":1697170867000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1365-2591.2005.00971.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,6,7]]},"references-count":21,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2005,7]]}},"alternative-id":["10.1111\/j.1365-2591.2005.00971.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1365-2591.2005.00971.x","archive":["Portico"],"relation":{},"ISSN":["0143-2885","1365-2591"],"issn-type":[{"value":"0143-2885","type":"print"},{"value":"1365-2591","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,6,7]]},"assertion":[{"value":"2005-06-07","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}