{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,15]],"date-time":"2026-11-15T07:19:18Z","timestamp":1794727158826,"version":"4.9.1"},"reference-count":22,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2005,8,9]],"date-time":"2005-08-09T00:00:00Z","timestamp":1123545600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int Endodontic J"],"published-print":{"date-parts":[[2005,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>Aim\u2002<\/jats:bold> To evaluate the cytotoxic effects of two brands of mineral trioxide aggregate (MTA) (Pro\u2010Root MTA<jats:sup>\u00ae<\/jats:sup> and MTA Angelus<jats:sup>\u00ae<\/jats:sup>) and Portland cement (PC) on the human ECV 304 endothelial cell line.<\/jats:p><jats:p><jats:bold>Methodology\u2002<\/jats:bold> Endothelial ECV 304 cells were incubated at 37\u2003\u00b0C in an atmosphere of 95% air, 5% carbon dioxide and 100% humidity for 7\u2003days and grown in F12 medium supplemented with 10% fetal bovine serum with 50\u2003<jats:italic>\u03bc<\/jats:italic>g\u2003mL<jats:sup>\u22121<\/jats:sup> of gentamicin sulphate. Effects of the materials on mitochondrial functions were measured by a colorimetric assay. At each experimental time interval (24, 48 and 72\u2003h), a dimethyl\u2010thiazol\u2010diphenyl tetrazolium bromid assay was conducted to measure cell viability. All assays were repeated three times to ensure reproducibility. Results were expressed as average absorbance (<jats:italic>A<\/jats:italic><jats:sub>570\\,nm<\/jats:sub>)\u2003\u00b1\u2003SD and the data were analysed statistically by one\u2010way analysis of variance and the Bonferroni post\u2010test. A <jats:italic>P<\/jats:italic>\u2010value &lt;0.05 was considered statistically significant.<\/jats:p><jats:p><jats:bold>Results\u2002<\/jats:bold> No statistically significant difference was shown between any of the experimental materials (<jats:italic>P<\/jats:italic>\u2003&gt;\u20030.05).<\/jats:p><jats:p><jats:bold>Conclusions\u2002<\/jats:bold> The two brands of MTA analysed, as well as the PC, initially showed a similar elevated cytotoxic effect that decreased gradually with time allowing the cell culture to become reestablished.<\/jats:p>","DOI":"10.1111\/j.1365-2591.2005.00987.x","type":"journal-article","created":{"date-parts":[[2005,8,9]],"date-time":"2005-08-09T16:54:40Z","timestamp":1123606480000},"page":"604-609","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":76,"title":["Cytotoxicity of MTA and Portland cement on human ECV 304 endothelial cells"],"prefix":"10.1111","volume":"38","author":[{"given":"G.","family":"De Deus","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Ximenes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E. 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