{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,31]],"date-time":"2023-10-31T20:42:39Z","timestamp":1698784959366},"reference-count":25,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2006,3,2]],"date-time":"2006-03-02T00:00:00Z","timestamp":1141257600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int Endodontic J"],"published-print":{"date-parts":[[2006,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>Aim\u2002<\/jats:bold> To determine whether commonly used endodontic sealers could either induce or increase the release of prostaglandin E<jats:sub>2<\/jats:sub> (PGE<jats:sub>2<\/jats:sub>) when in contact with cell types found in the periapical tissues.<\/jats:p><jats:p><jats:bold>Methodology\u2002<\/jats:bold> Freshly mixed samples of Roth 801 sealer, Sealapex<jats:sup>\u00ae<\/jats:sup> and ProRoot<jats:sup>\u00ae<\/jats:sup> mineral trioxide aggregate (MTA) were placed in contact with cultured macrophages and fibroblasts for 24\u2003h. The supernatant from the cultures was assayed for PGE<jats:sub>2<\/jats:sub> using enzyme\u2010linked immunosorbent assay. Cell viability counts were made. As a positive control, similar cultures were also exposed to lipopolysaccharide and the supernatant analysed for PGE<jats:sub>2<\/jats:sub>. Data were compared by <jats:sc>anova<\/jats:sc>.<\/jats:p><jats:p><jats:bold>Results\u2002<\/jats:bold> The three materials examined in these experiments did not stimulate increased PGE<jats:sub>2<\/jats:sub> release from either of the cell lines. In control cultures, lipolysaccharide increased PGE<jats:sub>2<\/jats:sub> release from macrophages but not from fibroblasts. Viability counts revealed that, whilst Roth 801 sealer caused some cell death in both fibroblasts and macrophages, Sealapex<jats:sup>\u00ae<\/jats:sup> led to cell death only in the macrophage cultures. ProRoot<jats:sup>\u00ae<\/jats:sup> MTA did not lead to statistically significant cell death in either culture.<\/jats:p><jats:p><jats:bold>Conclusions\u2002<\/jats:bold> Under 24\u2010h culture conditions, the three freshly mixed test materials did not increase directly either production or release of PGE<jats:sub>2<\/jats:sub> from either macrophages or gingival fibroblasts. Roth 801 decreased cell viability counts for both fibroblasts and macrophages. Sealapex<jats:sup>\u00ae<\/jats:sup> decreases macrophage viability. ProRoot<jats:sup>\u00ae<\/jats:sup> MTA did not affect viability in either cell line.<\/jats:p>","DOI":"10.1111\/j.1365-2591.2006.01070.x","type":"journal-article","created":{"date-parts":[[2006,3,2]],"date-time":"2006-03-02T15:51:33Z","timestamp":1141314693000},"page":"357-362","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Prostaglandin E<sub>2<\/sub> production and viability of cells cultured in contact with freshly mixed endodontic materials"],"prefix":"10.1111","volume":"39","author":[{"given":"K. K.","family":"Melegari","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T. M.","family":"Botero","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G. 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