{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T11:29:24Z","timestamp":1790335764347,"version":"4.1.0"},"reference-count":35,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2008,9,10]],"date-time":"2008-09-10T00:00:00Z","timestamp":1221004800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int Endodontic J"],"published-print":{"date-parts":[[2008,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>Aim\u2002<\/jats:bold> To investigate the effects of substance P (SP) on gelatinolytic activity of matrix metalloproteinases (MMPs) in human pulp cells.<\/jats:p><jats:p><jats:bold>Methodology\u2002<\/jats:bold> Human dental pulp cells were isolated and cultured. Subconfluent cells, between the third and sixth passages, were maintained under serum deprivation for 18\u2003h followed by the treatment of varying doses of SP (1\u2003pmol\u2003L<jats:sup>\u22121<\/jats:sup>, 100\u2003pmol\u2003L<jats:sup>\u22121<\/jats:sup>, 10\u2003nmol\u2003L<jats:sup>\u22121<\/jats:sup>, 1\u2003\u03bcmol\u2003L<jats:sup>\u22121<\/jats:sup> and 100\u2003\u03bcmol\u2003L<jats:sup>\u22121<\/jats:sup>). Conditioned media were then subjected to gelatin zymography using 8% sodium dodecyl sulphate polyacrylamide gel electrophoresis minigels containing 1.5\u2003g\u2003L<jats:sup>\u22121<\/jats:sup> gelatin. The effect of SP on intracellular reactive oxygen species (ROS) was also examined by confocal microscopy. ROS scavenger <jats:italic>N<\/jats:italic>\u2010Acetyl\u2010<jats:sc>l<\/jats:sc>\u2010cysteine (NAC, 5\u2003mmol\u2003L<jats:sup>\u22121<\/jats:sup>) was utilized to evaluate the roles of ROS pathway in mediating the impact of SP on cellular gelatinolytic activity. Data were analysed using analysis of variance with Bonferroni correction for multiple comparisons or an unpaired Student\u2019s <jats:italic>t<\/jats:italic>\u2010test.<\/jats:p><jats:p><jats:bold>Results\u2002<\/jats:bold> Substance P, at levels above 1\u2003\u03bcmol\u2003L<jats:sup>\u22121<\/jats:sup>, remarkably enhanced MMP\u20102 activity reflected by the band migrating at 66\u2003kDa (<jats:italic>P\u2003<\/jats:italic>&lt;<jats:italic>\u2003<\/jats:italic>0.05). A gelatinolytic band at approximately 44\u2003kDa appeared to be intensified in a SP dose\u2010dependent manner. In addition, it was demonstrated that SP could induce ROS production in pulp cells and ROS scavenger NAC was further found to significantly reduce MMP\u20102 activity (<jats:italic>P\u2003<\/jats:italic>&lt;<jats:italic>\u2003<\/jats:italic>0.05), as well as other bands of gelatinolytic proteinases.<\/jats:p><jats:p><jats:bold>Conclusion\u2002<\/jats:bold> Substance P can influence gelatinolytic activity in human pulp cells via ROS pathway.<\/jats:p>","DOI":"10.1111\/j.1365-2591.2008.01437.x","type":"journal-article","created":{"date-parts":[[2008,9,10]],"date-time":"2008-09-10T11:35:19Z","timestamp":1221046519000},"page":"856-862","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Substance P influenced gelatinolytic activity via reactive oxygen species in human pulp 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