{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T14:47:08Z","timestamp":1790866028675,"version":"4.1.0"},"reference-count":30,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2011,9,8]],"date-time":"2011-09-08T00:00:00Z","timestamp":1315440000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int Endodontic J"],"published-print":{"date-parts":[[2012,1]]},"abstract":"<jats:sec><jats:label\/><jats:p><jats:bold>Scelza MZ, Linhares AB, da Silva LE, Granjeiro JM, Alves GG.<\/jats:bold> A multiparametric assay to compare the cytotoxicity of endodontic sealers with primary human osteoblasts. <jats:italic>International Endodontic Journal<\/jats:italic>, <jats:bold>45<\/jats:bold>, 12\u201318, 2012.<\/jats:p><\/jats:sec><jats:sec><jats:title>Abstract<\/jats:title><jats:p><jats:bold>Aim\u2002<\/jats:bold> To compare the cytotoxicity of four endodontic sealers (Sealapex, Pulp Canal Sealer EWT, Real Seal and MTA Fillapex) either 1 or 7\u2003days after mixing, when assessed through a multiparametric analysis employing human primary cells closely related to periapical tissues.<\/jats:p><jats:p><jats:bold>Methodology\u2002<\/jats:bold> Extracts of each sealer were prepared following 24\u2010h exposure to culture media, at either 24\u2003h or 7\u2003days after mixing. Primary human osteoblasts were exposed to extracts for 24\u2003h, at 37\u2003\u00b0C with 5% CO<jats:sub>2<\/jats:sub>, and cell viability was evaluated by a multiparametric assay assessing sequentially, on the same cells, mitochondrial activity (XTT), membrane integrity (neutral red test) and total cell density (crystal violet dye exclusion test). Results from each test and experimental time were compared by 2\u2010way analysis of variance (<jats:sc>anova<\/jats:sc>).<\/jats:p><jats:p><jats:bold>Results\u2002<\/jats:bold> All endodontic sealers had strong cytotoxicity 24\u2003h after mixing, according to all parameters evaluated. At a longer setting period (7\u2003days), viability for Sealapex was significantly increased (<jats:italic>P<\/jats:italic>\u2003&lt;\u20030.05) and Pulp Canal Sealer achieved levels of cytocompatibility similar to the control group. The <jats:sc>anova<\/jats:sc> indicated a general correlation between the cytotoxicity of the materials and the time after mixing, with some level of dependence on the cell viability assay employed.<\/jats:p><jats:p><jats:bold>Conclusions\u2002<\/jats:bold> All materials had high cytotoxic levels for human primary cells, mostly on a time\u2010dependent basis, as shown by three different cell viability tests.<\/jats:p><\/jats:sec>","DOI":"10.1111\/j.1365-2591.2011.01941.x","type":"journal-article","created":{"date-parts":[[2011,9,8]],"date-time":"2011-09-08T16:16:02Z","timestamp":1315498562000},"page":"12-18","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":72,"title":["A multiparametric assay to compare the cytotoxicity of endodontic sealers with primary human osteoblasts"],"prefix":"10.1111","volume":"45","author":[{"given":"M. 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