{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T15:31:35Z","timestamp":1764603095094},"reference-count":35,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2012,3,27]],"date-time":"2012-03-27T00:00:00Z","timestamp":1332806400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int Endodontic J"],"published-print":{"date-parts":[[2012,9]]},"abstract":"<jats:p><jats:bold>Silva EJNL, Herrera DR, Almeida JFA, Ferraz CCR, GomesBPFA, Zaia AA.<\/jats:bold> Evaluation of cytotoxicity and up\u2010regulation of gelatinases in fibroblast cells by three root repair materials. <jats:italic>International Endodontic Journal<\/jats:italic>,\u00a0<jats:bold>45<\/jats:bold>, 815\u2013820, 2012.<\/jats:p><jats:p><jats:bold>Aim\u2002<\/jats:bold> To investigate the effects of root repair materials on the cytotoxicity and gelatinolytic activity of matrix metalloproteinases (MMPs) in 3T3 fibroblasts.<\/jats:p><jats:p><jats:bold>Methodology\u2002<\/jats:bold> Fibroblasts (3T3, 3\u2003\u00d7\u200310<jats:sup>5<\/jats:sup>\u2003cells per well) were incubated with elutes of calcium hydroxide (Biodin\u00e2mica, Ibipor\u00e3, PR, Brazil), EndoBinder (Binderware, S\u00e3o Carlos, SP, Brazil) and mineral trioxide aggregate (MTA) (Angelus, Londrina, PR, Brazil) for 24\u2003h. The cytotoxicity of all root repair materials was determined using the MTT assay. Supernatants of cell cultures incubated with materials were collected after 24\u2003h to determine the levels of MMP\u20102 and MMP\u20109 gelatinolytic activity by gelatin zymography. Data were analysed using <jats:sc>anova<\/jats:sc> and Tukey\u2019s test.<\/jats:p><jats:p><jats:bold>Results\u2002<\/jats:bold> Cells secreted MMP\u20102 after 24\u2003h with calcium hydroxide inducing significantly greater MMP\u20102 expression in relation to the control and the other root repair materials (<jats:italic>P<\/jats:italic>\u2003&lt;\u20030.05). The cytotoxicity results revealed that there was no significant difference in the cell viability of MTA, EndoBinder and the control group. However, there was a significantly reduced cell viability of 3T3 fibroblasts in association with calcium hydroxide (<jats:italic>P<\/jats:italic>\u2003&lt;\u20030.05).<\/jats:p><jats:p><jats:bold>Conclusions\u2002<\/jats:bold> Calcium hydroxide was associated with significantly less cell viability when compared with EndoBinder and MTA. All materials had gelatinolytic activity for MMP\u20102 with calcium hydroxide being associated with the greatest activity.<\/jats:p>","DOI":"10.1111\/j.1365-2591.2012.02038.x","type":"journal-article","created":{"date-parts":[[2012,3,5]],"date-time":"2012-03-05T15:34:46Z","timestamp":1330961686000},"page":"815-820","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Evaluation of cytotoxicity and up\u2010regulation of gelatinases in fibroblast cells by three root repair materials"],"prefix":"10.1111","volume":"45","author":[{"given":"E. J. N. 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