{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T05:18:03Z","timestamp":1792646283216,"version":"4.5.0"},"reference-count":32,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2012,7,3]],"date-time":"2012-07-03T00:00:00Z","timestamp":1341273600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int Endodontic J"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:sec>\n                    <jats:label\/>\n                    <jats:p>\n                      <jats:bold>Pereira LO, Rubini MR, Silva JR, Oliveira DM, Silva ICR, Po\u00e7as\u2010Fonseca MJ, Azevedo RB.<\/jats:bold>\n                      Comparison of stem cell properties of cells isolated from normal and inflamed dental pulps.\n                      <jats:italic>International Endodontic Journal<\/jats:italic>\n                      00, 000\u2013000, 2012.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Abstract<\/jats:title>\n                    <jats:p>\n                      <jats:bold>Aim\u2002<\/jats:bold>\n                      To compare cells from normal and inflamed human dental pulps regarding the presence of stem cells, their proliferation and differentiation potential.\n                    <\/jats:p>\n                    <jats:p>\n                      <jats:bold>Methodology\u2002<\/jats:bold>\n                      Human dental pulp stem cells (hDPSCs) were isolated from normal (DPSC\u2010N) and inflamed dental pulps (DPSC\u2010I). They were compared in respect to proliferation (MTT assay), morphology and STRO\u20101 expression. STRO\u20101\u2010positive cells were subject to proliferation (MTT and CFU counting) and morphological analyses and then submitted to odonto\u2010osteogenic, adipogenic and condrogenic differentiation. Differentiated cells were evaluated concerning morphology and the expression, by qRT\u2010PCR, of\n                      <jats:italic>BSP<\/jats:italic>\n                      ,\n                      <jats:italic>LPL<\/jats:italic>\n                      and\n                      <jats:italic>SOX\u20109<\/jats:italic>\n                      genes. The amount of mineralized matrix produced after odonto\u2010osteogenic differentiation was compared with quantitative Alizarin Red staining.\n                    <\/jats:p>\n                    <jats:p>\n                      <jats:bold>Results\u2002<\/jats:bold>\n                      No difference was observed in the morphology and in the proliferation rate of DPSC\u2010N and DPSC\u2010I either before or after separation of STRO\u20101\u2010positive cells. These cells represented 0.46% (\u00b10.14) and 0.43% (\u00b10.19) of the cell population from normal and inflamed dental pulps, respectively. Both DPSC\u2010N and DPSC\u2010I were capable of differentiating under the three assayed conditions and presented similar patterns for\n                      <jats:italic>BSP<\/jats:italic>\n                      ,\n                      <jats:italic>LPL<\/jats:italic>\n                      and\n                      <jats:italic>SOX\u20109<\/jats:italic>\n                      expression. Mineralized matrix production was also compatible. In all the quantitative experiments, differences were found between cells from each patient, either from normal or from inflamed pulps. Nonetheless, there was no statistical difference between these two groups.\n                    <\/jats:p>\n                    <jats:p>\n                      <jats:bold>Conclusion\u2002<\/jats:bold>\n                      The morphology, proliferation rate and differentiation potential of DPSC\u2010I were similar to the observed in DPSC\u2010N, thus demonstrating that the inflammatory process did not affect the stem cell properties that were assessed.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/j.1365-2591.2012.02068.x","type":"journal-article","created":{"date-parts":[[2012,4,17]],"date-time":"2012-04-17T13:41:09Z","timestamp":1334670069000},"page":"1080-1090","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":61,"title":["Comparison of stem cell properties of cells isolated from normal and inflamed dental pulps"],"prefix":"10.1111","volume":"45","author":[{"given":"L. 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