{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T09:33:22Z","timestamp":1789205602844,"version":"build-2803163510"},"reference-count":12,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2012,6,6]],"date-time":"2012-06-06T00:00:00Z","timestamp":1338940800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int Endodontic J"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:sec>\n                    <jats:label\/>\n                    <jats:p>\n                      <jats:bold>Yonehiro J, Yamashita A, Yoshida Y, Yoshizawa S, Ohta K, Kamata N, Okihara T, Nishimura F.<\/jats:bold>\n                      Establishment of an\n                      <jats:italic>ex vivo<\/jats:italic>\n                      pulpitis model by co\u2010culturing immortalized dental pulp cells and macrophages.\n                      <jats:italic>International Endodontic Journal.<\/jats:italic>\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Abstract<\/jats:title>\n                    <jats:p>\n                      <jats:bold>Aim\u2002<\/jats:bold>\n                      To establish an\n                      <jats:italic>ex vivo<\/jats:italic>\n                      pulpitis model by co\u2010culturing dental pulp cells with macrophages.\n                    <\/jats:p>\n                    <jats:p>\n                      <jats:bold>Methodology\u2002<\/jats:bold>\n                      As dental pulp cells, immortalized human dental pulp cells, named DP\u20101, were used, whilst as macrophage cell lines, the differentiated human monocytic cell line, THP\u20101, was used. In some experiments, primary dental pulp cells were isolated and used to confirm the results obtained in the experiments using immortalized cells. Co\u2010culturing was performed using transwell systems. Inflammatory responses were evaluated by measuring cytokines produced by the cells.\n                    <\/jats:p>\n                    <jats:p>\n                      <jats:bold>Results\u2002<\/jats:bold>\n                      Co\u2010culturing both cell types markedly up\u2010regulated inflammatory cytokine production as compared with the cells cultured independently, suggesting that both cell types interact with each other to synergistically produce higher amounts of inflammatory cytokines. Interestingly, both DP\u20101 and primary dental pulp cells appeared to produce molecules stimulating macrophages to produce tumour necrosis factor\u2010\u03b1\u2010.\n                    <\/jats:p>\n                    <jats:p>\n                      <jats:bold>Conclusion\u2002<\/jats:bold>\n                      Co\u2010culturing immortalized dental pulp cells and macrophages may be a new\n                      <jats:italic>ex vivo<\/jats:italic>\n                      model for studying the pathophysiology of reversible pulpitis.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/j.1365-2591.2012.02074.x","type":"journal-article","created":{"date-parts":[[2012,5,1]],"date-time":"2012-05-01T22:45:52Z","timestamp":1335912352000},"page":"1103-1108","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Establishment of an\n                    <i>ex vivo<\/i>\n                    pulpitis model by co\u2010culturing immortalized dental pulp cells and macrophages"],"prefix":"10.1111","volume":"45","author":[{"given":"J.","family":"Yonehiro","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Yamashita","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Yoshida","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Yoshizawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Ohta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"Kamata","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Okihara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Nishimura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,6,6]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-323-06489-7.00001-1"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra052723"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-323-06489-7.00013-8"},{"key":"e_1_2_7_5_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.4049\/jimmunol.156.1.1","article-title":"Matrix metalloproteinases in immunity","volume":"156","author":"Goetzl EJ","year":"1996","journal-title":"Journal of Immunology"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI116988"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0714.2004.00228.x"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2591.2007.01215.x"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.7.4102-4108.2004"},{"key":"e_1_2_7_10_1","first-page":"1388","article-title":"Expression of tissue transglutaminase in cultured monocytic leukaemia (THP\u20101) cells during differentiation","volume":"46","author":"Mehta K","year":"1986","journal-title":"Cancer Research"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2009.04.005"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/jlb.59.4.555"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1834-7819.2004.tb00059.x"}],"container-title":["International Endodontic Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1365-2591.2012.02074.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1365-2591.2012.02074.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,30]],"date-time":"2023-10-30T07:59:42Z","timestamp":1698652782000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1365-2591.2012.02074.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,6,6]]},"references-count":12,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2012,12]]}},"alternative-id":["10.1111\/j.1365-2591.2012.02074.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1365-2591.2012.02074.x","archive":["Portico"],"relation":{},"ISSN":["0143-2885","1365-2591"],"issn-type":[{"value":"0143-2885","type":"print"},{"value":"1365-2591","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,6,6]]},"assertion":[{"value":"2012-06-06","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}