{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T20:25:09Z","timestamp":1790367909559,"version":"4.1.0"},"reference-count":22,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2012,7,7]],"date-time":"2012-07-07T00:00:00Z","timestamp":1341619200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society of Promotion of Science","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int Endodontic J"],"published-print":{"date-parts":[[2013,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>To find possible reagents to minimize inflammatory responses by using an established pulpitis models for the purpose of developing new pulp\u2010capping materials, and to test the possible use of phosphorylated pullulan as a carrier for such an anti\u2010inflammatory reagent.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methodology<\/jats:title><jats:p>Co\u2010culturing was performed using transwell systems. Inflammatory responses were evaluated by measuring cytokines produced by the cells. The effects of two flavonoids, luteolin and quercetin, as anti\u2010inflammatory reagents, and phosphorylated pullulan, which potentially achieves a sufficient marginal sealing to hydroxyapatite and slowly releases luteolin, as a carrier for flavonoids, were tested.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Flavonols, particularly luteolin, dramatically attenuated inflammatory cytokine production, which was augmented by co\u2010cultures. Luteolin was successfully enclosed by phosphorylated pullulan. Finally, it was confirmed that luteolin released from phosphorylated pullulan was effective in reducing cytokine production by co\u2010cultures.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Combination of phosphorylated pullulan and luteolin could be potentially used in the treatment of dental pulp inflammation.<\/jats:p><\/jats:sec>","DOI":"10.1111\/j.1365-2591.2012.02095.x","type":"journal-article","created":{"date-parts":[[2012,6,7]],"date-time":"2012-06-07T08:47:43Z","timestamp":1339058863000},"page":"119-127","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Flavonol\u2010containing phosphorylated pullulan may attenuate pulp inflammation"],"prefix":"10.1111","volume":"46","author":[{"given":"J.","family":"Yonehiro","sequence":"first","affiliation":[{"name":"Department of Dental Science for Health Promotion Hiroshima University Graduate School of Biomedical Sciences Hiroshima Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Yoshida","sequence":"additional","affiliation":[{"name":"Department of Biomaterials Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences Okayama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Yamashita","sequence":"additional","affiliation":[{"name":"Department of Dental Science for Health Promotion Hiroshima University Graduate School of Biomedical Sciences Hiroshima Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Yoshizawa","sequence":"additional","affiliation":[{"name":"Center for Craniofacial Regeneration Department of Oral Biology School of Dental Medicine University of Pittsburgh Pittsburg PA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Ohta","sequence":"additional","affiliation":[{"name":"Department of Oral Surgery Hiroshima University Graduate School of Biomedical Science Hiroshima Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"Kamata","sequence":"additional","affiliation":[{"name":"Department of Oral Surgery Hiroshima University Graduate School of Biomedical Science Hiroshima Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Okihara","sequence":"additional","affiliation":[{"name":"Department of Material Chemistry Okayama University Graduate School of Natural Science and Technology Okayama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Nishimura","sequence":"additional","affiliation":[{"name":"Department of Dental Science for Health Promotion Hiroshima University Graduate School of Biomedical Sciences Hiroshima Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,7,7]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0031-9422(99)00286-1"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-3083.2003.01213.x"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.14219\/jada.archive.2008.0160"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1021624520740"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200425778"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf1034306"},{"key":"e_1_2_7_8_1","first-page":"2916","article-title":"Flavonoids, dietary\u2010derived inhibitors of cell proliferation and in vitro angiogenesis","volume":"57","author":"Fotsis T","year":"1997","journal-title":"Cancer Research"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2008.05.013"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0163-7258(02)00298-X"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2004.10.002"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0714.2004.00228.x"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.21098"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2008.01.013"},{"key":"e_1_2_7_15_1","first-page":"1388","article-title":"Expression of tissue transglutaminase in cultured monocytic leukemia (THP\u20101) cells during differentiation","volume":"46","author":"Mehta K","year":"1986","journal-title":"Cancer Research"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1590\/S1678-77572009000600002"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0031-9422(00)84689-0"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/mabi.200600053"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dental.2010.10.023"},{"key":"e_1_2_7_20_1","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1016\/S0022-3565(24)29679-8","article-title":"Luteolin inhibits an endotoxin\u2010stimulated phosphorylation cascade and proinflammatory cytokine production in macrophages","volume":"296","author":"Xagorari A","year":"2001","journal-title":"Journal of Pharmacology and Experimental Therapeutics"},{"key":"e_1_2_7_21_1","article-title":"Establishment of in vitro pulpitis model by co\u2010culturing pulp cells with macrophages","author":"Yonehiro J","year":"2011","journal-title":"International Endodontic Journal"},{"key":"e_1_2_7_22_1","unstructured":"YoshidaY TanakaM SuzukiKet\u00a0al. (2011)Kit for adhering biological hard tissues. WO 2011\/102530."},{"key":"e_1_2_7_23_1","first-page":"195","article-title":"Quercetin exerts a neuroprotective effect through inhibition of the iNOS\/NO system and pro\u2010inflammation gene expression in PC12 cells and in zebrafish","volume":"27","author":"Zhang ZJ","year":"2010","journal-title":"International Journal of Molecular Medicine"}],"container-title":["International Endodontic Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1365-2591.2012.02095.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1365-2591.2012.02095.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,29]],"date-time":"2025-03-29T21:57:21Z","timestamp":1743285441000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1365-2591.2012.02095.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,7,7]]},"references-count":22,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2013,2]]}},"alternative-id":["10.1111\/j.1365-2591.2012.02095.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1365-2591.2012.02095.x","archive":["Portico"],"relation":{},"ISSN":["0143-2885","1365-2591"],"issn-type":[{"value":"0143-2885","type":"print"},{"value":"1365-2591","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,7,7]]},"assertion":[{"value":"2011-12-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-06-01","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-07-07","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}