{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T09:07:34Z","timestamp":1791104854729,"version":"4.1.0"},"reference-count":36,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2012,8,21]],"date-time":"2012-08-21T00:00:00Z","timestamp":1345507200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int Endodontic J"],"published-print":{"date-parts":[[2013,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>To analyse and compare the expression of nemosis markers in human lung and dental pulp fibroblasts and to determine whether this process differs by the type of mesenchymal cell.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Human dental pulp fibroblasts were obtained from unerupted third molars. Sound lung and pulpal fibroblasts were cultured\n                      <jats:italic>in vitro<\/jats:italic>\n                      as spheroids to determine the expression of the nemosis hallmark cyclooxygenase\u20102 (\n                      <jats:styled-content style=\"fixed-case\">COX<\/jats:styled-content>\n                      \u20102)\n                      <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content>\n                      using RT\u2010PCR and the concentrations of vascular endothelial growth factor (\n                      <jats:styled-content style=\"fixed-case\">VEGF<\/jats:styled-content>\n                      ) and hepatocyte growth factor\/scatter factor (\n                      <jats:styled-content style=\"fixed-case\">HGF<\/jats:styled-content>\n                      \/\n                      <jats:styled-content style=\"fixed-case\">SF<\/jats:styled-content>\n                      ) proteins using an\n                      <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>\n                      test. Cell viability within spheroids was also compared with spheroid diameters over time.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Increased expression of\n                      <jats:styled-content style=\"fixed-case\">COX<\/jats:styled-content>\n                      \u20102 and\n                      <jats:styled-content style=\"fixed-case\">VEGF<\/jats:styled-content>\n                      was found in all spheroids compared with corresponding monolayers. Although\n                      <jats:styled-content style=\"fixed-case\">HGF<\/jats:styled-content>\n                      \/\n                      <jats:styled-content style=\"fixed-case\">SF<\/jats:styled-content>\n                      was highly expressed in\n                      <jats:styled-content style=\"fixed-case\">MRC<\/jats:styled-content>\n                      5 cells, dental pulp fibroblasts aggregates maintained only a basal level compared with monolayer cultures. Further, the observed progressive loss of viable cells explained the decreased diameters of spheroids over time. The results demonstrate that nemosis occurs in sound lung and pulpal fibroblasts. This cell death also displays differences between these two different cell types, as they do not produce the same growth factors quantity release.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      The nemosis process occurred in human dental pulp fibroblasts and is different between the two cell types studied. This\n                      <jats:italic>in vitro<\/jats:italic>\n                      experimental nemosis model could become an interesting inflammatory tool. More investigations are needed to compare nemosis process in dental pulp fibroblast and inflammation during pulpitis.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/j.1365-2591.2012.02114.x","type":"journal-article","created":{"date-parts":[[2012,7,11]],"date-time":"2012-07-11T12:05:56Z","timestamp":1342008356000},"page":"308-316","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Characterization of a programmed necrosis process in 3\u2010dimensional cultures of dental pulp fibroblasts"],"prefix":"10.1111","volume":"46","author":[{"given":"J.","family":"Le Clerc","sequence":"first","affiliation":[{"name":"Facult\u00e9 d'Odontologie Laboratoire de Biomat\u00e9riaux en Site Osseux, UMR CNRS 6226, Sciences Chimiques de Rennes, Universit\u00e9 de Rennes 1 Rennes France"},{"name":"Service d'Odontologie Conservatrice et Endodontie, Centre Hospitalier Universitaire Rennes 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