{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T22:14:23Z","timestamp":1793052863169,"version":"4.5.0"},"reference-count":37,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2006,3,27]],"date-time":"2006-03-27T00:00:00Z","timestamp":1143417600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int J of Andrology"],"published-print":{"date-parts":[[2006,10]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>For artificial insemination (AI) in pigs, preservation of liquid boar semen at 16\u201320\u2003\u00b0C is still common practice as sperm cryopreservation remains suboptimal in this species. To meet the different needs of the swine industry, several extenders have been developed to preserve semen in liquid form for short\u2010 and long\u2010term storage. In the present study, three different commercial extenders devised for short\u2010term (BTS+) or long\u2010term preservation (MR\u2010A and X\u2010Cell), were used to test whether storage of semen from four mature, fertile boars at 17\u2003\u00b0C for 96\u2003h would affect sperm characteristics relevant for fertility, such as motility, membrane integrity and chromatin stability. Computer\u2010assisted sperm analysis, and stainings with the acylated membrane dye SYBR\u201014\/propidium iodide, and acridine orange in connection with flow cytometry were used to evaluate these variables. Percentages of total motile spermatozoa decreased slightly, but significantly, after 72\u201396\u2003h. While membrane integrity values varied during the period of study, no significant changes in either membrane integrity or chromatin stability were, however, registered. This suggests a customary 96\u2010day storage at 17\u2003\u00b0C in these extenders was too short an interval to cause losses of integrity in nuclear DNA in the boar population studied.<\/jats:p>","DOI":"10.1111\/j.1365-2605.2006.00694.x","type":"journal-article","created":{"date-parts":[[2006,3,27]],"date-time":"2006-03-27T11:46:44Z","timestamp":1143460004000},"page":"543-552","source":"Crossref","is-referenced-by-count":66,"title":["Effect of storage in short\u2010 and long\u2010term commercial semen extenders on the motility, plasma membrane and chromatin integrity of boar spermatozoa"],"prefix":"10.1111","volume":"29","author":[{"given":"Marco","family":"De Ambrogi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Ballester","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando","family":"Saravia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ignacio","family":"Caballero","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anders","family":"Johannisson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margareta","family":"Wallgren","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magnus","family":"Andersson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heriberto","family":"Rodriguez\u2010Martinez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,3,27]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod27.5.1102"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(98)00016-8"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2003.10.019"},{"key":"e_1_2_6_5_1","first-page":"518","article-title":"A study on the technique of hemocytometric determination of sperm motility and sperm concentration in bull semen","volume":"42","author":"Bane A.","year":"1952","journal-title":"Cornell Veterinarian"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2004.09.006"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2004.01.015"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2003.12.006"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4320(00)00171-8"},{"key":"e_1_2_6_10_1","volume-title":"Current Protocols in Cytometry","author":"Evenson D. 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