{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T05:20:46Z","timestamp":1767849646463,"version":"3.49.0"},"reference-count":38,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2006,5,26]],"date-time":"2006-05-26T00:00:00Z","timestamp":1148601600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int J of Andrology"],"published-print":{"date-parts":[[2006,12]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Spermatozoa from some boars sustain the process of cryopreservation poorly and yield poor fertility after artificial insemination. Poor freezability has not been disclosed using conventional semen analyses. A defective chromatin can, if present in a substantial number of spermatozoa, affect the fertilizing ability of spermatozoa. Here we tested the hypothesis that nuclear DNA instability could explain differences in freezability among boars, and complement or supersede conventional tests for sperm quality such as sperm motility or membrane assessments. Frozen\u2013thawed (FT) spermatozoa from a total of 44 stud boars were assessed by the sperm chromatin structure assay (SCSA), in relation to computer\u2010assisted sperm analysis\u2010derived sperm motility variables and sperm viability (triple fluorescent microscopic staining), including three experiments. The first trial, including 24 boars, evaluated the relationship between the sperm motility and viability with levels of DNA integrity. The SCSA showed that most spermatozoa had intact DNA [levels of DNA fragmentation index (%DFI) ranging from 0.63% to 11.85%] significantly correlated (albeit weakly) with current sperm quality variables. The second trial, on 15 boars, assessed the influence of two different thawing rates (20\u2003s at 37\u2003\u00b0C vs. 8\u2003s at 70\u2003\u00b0C) and the post\u2010thaw incubation times (0, 30, 150 and 300\u2003min) at 37\u2003\u00b0C on FT\u2010boar sperm quality. The highest sperm survival (<jats:italic>p<\/jats:italic>\u2003&lt;\u20030.05) and the lowest DNA damage (<jats:italic>p<\/jats:italic>\u2003&lt;\u20030.01) were achieved when thawing was carried out at 70\u2003\u00b0C for 8\u2003s, without any change during the first 150\u2003min of incubation. Finally, the third experiment studied if differences in sperm freezability showed by stud boar semen, as \u2018good\u2019 or \u2018bad\u2019 freezers by conventional analyses, could be attributed to differences in chromatin structure. All SCSA parameters were low, but significantly (<jats:italic>p<\/jats:italic>\u2003&lt;\u20030.05\u20130.001) higher for \u2018bad\u2019 freezers, showing they had less homogeneous sperm chromatin than the \u2018good\u2019 freezers. The results indicate that SCSA outcome complements conventional assessment of FT\u2010boar spermatozoa, disclosing differences in their ability to sustain freezing and thawing. However, the low overall DNA damage observed in FT spermatozoa seems to have poor biological significance.<\/jats:p>","DOI":"10.1111\/j.1365-2605.2006.00699.x","type":"journal-article","created":{"date-parts":[[2006,5,26]],"date-time":"2006-05-26T14:33:36Z","timestamp":1148654016000},"page":"583-591","source":"Crossref","is-referenced-by-count":60,"title":["Differences in SCSA outcome among boars with different sperm freezability"],"prefix":"10.1111","volume":"29","author":[{"given":"Marta","family":"Hern\u00e1ndez","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jordi","family":"Roca","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joan","family":"Ballester","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan M.","family":"V\u00e1zquez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emilio A.","family":"Mart\u00ednez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anders","family":"Johannisson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernando","family":"Saravia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heriberto","family":"Rodr\u00edguez\u2010Mart\u00ednez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,5,26]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod60.1.32"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD03089"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-2795(200003)55:3<282::AID-MRD6>3.0.CO;2-7"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(01)00588-X"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2004.09.006"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2004.08.004"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.2164\/jandrol.04056"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939-4640.2004.tb02805.x"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.2164\/jandrol.05068"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(02)00701-X"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2005.05.011"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2005.05.044"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2005.09.011"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0093-691X(94)90174-H"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939-4640.2002.tb02599.x"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.2005.00626.x"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2004.11.024"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(03)02212-X"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod43.1.55"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2004.08.001"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2004.09.018"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939-4640.2000.tb03273.x"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(01)00456-3"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4320(00)00157-3"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2005.05.056"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/15.8.1717"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2005.06.019"},{"key":"e_1_2_7_29_1","first-page":"519","article-title":"Boar semen evaluation in practice","volume":"31","author":"Martin Rillo S.","year":"1996","journal-title":"Reproduction in Domestic Animals"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939-4640.2004.tb02782.x"},{"key":"e_1_2_7_31_1","first-page":"261","article-title":"Strategies to improve the fertility of frozen\u2010thawed boar semen for artificial insemination","author":"Roca J.","year":"2006","journal-title":"Control of Pig Reproduction"},{"key":"e_1_2_7_32_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.17221\/5668-VETMED","article-title":"Bull and boar sperm DNA integrity evaluated by sperm chromatin structure assay in the Czech Republic","volume":"49","author":"Rybar R.","year":"2004","journal-title":"Czech Republic Veterinary Medicine"},{"key":"e_1_2_7_33_1","first-page":"243","article-title":"Secretory activity of boar seminal vesicle glands","volume":"2","author":"Strzezek J.","year":"2002","journal-title":"Reproductive Biology"},{"key":"e_1_2_7_34_1","first-page":"355","article-title":"Sources of variation in boar spermatozoa fertility following cryopreservation","volume":"39","author":"Thurston L. M.","year":"1999","journal-title":"Cryobiology"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod66.3.545"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4320(00)00099-3"},{"key":"e_1_2_7_37_1","first-page":"261","article-title":"Zur Tierfgefrierung von Ebersperma: Labor und besamungsergebnisse mit dem H\u00fclsenberger Pailleten Verfahren","volume":"82","author":"Westendorf P.","year":"1975","journal-title":"Dtch Tier\u00e1rztl Wschr"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.1995.tb00019.x"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(00)01796-9"}],"container-title":["International Journal of Andrology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1365-2605.2006.00699.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1365-2605.2006.00699.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,12]],"date-time":"2023-10-12T23:18:34Z","timestamp":1697152714000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1365-2605.2006.00699.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,5,26]]},"references-count":38,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2006,12]]}},"alternative-id":["10.1111\/j.1365-2605.2006.00699.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1365-2605.2006.00699.x","archive":["Portico"],"relation":{},"ISSN":["0105-6263","1365-2605"],"issn-type":[{"value":"0105-6263","type":"print"},{"value":"1365-2605","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,5,26]]}}}