{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,7,31]],"date-time":"2024-07-31T17:51:41Z","timestamp":1722448301967},"reference-count":24,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2007,1,22]],"date-time":"2007-01-22T00:00:00Z","timestamp":1169424000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Experimental Path"],"published-print":{"date-parts":[[2007,2]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Mina\u00e7u virus was isolated from <jats:italic>Ochlerotatus scapularis<\/jats:italic> (Diptera: Culicidae) in Mina\u00e7u, Goi\u00e1s State, Brazil, in 1996. In attempting characterization of virus serological (hemagluttination inhibition, HI; indirect immunofluorescence assay, IFA), physicochemical [test for deoxycholate acid (DCA) sensitivity; polyacrylamide gel electrophoresis (PAGE)] tests and ultrastructural studies were made. Virus was also assayed in suckling mice after intracerebral inoculation of 0.02\u2003ml and in VERO and C6\/36 cells with 0.1\u2003ml of viral suspension containing 10<jats:sup>5<\/jats:sup> LD<jats:sub>50<\/jats:sub>\/ml. Inoculated and control systems were observed daily. Every 24\u2003h, one control and two inoculated animals were killed for tissue testing, including histopathological changes by haematoxylin and eosin (HE)\u2010stained sections, which were semi\u2010quantified. Research into viral antigen in the tissues of mice [central nervous system (CNS), liver, heart, lungs, spleen and kidneys] was carried out by the immunohistochemical technique using the peroxidase system. The virus only replicated in VERO cells, with antigen positive by IFA. Positive complement fixation tests were only obtained using antiserum of Mina\u00e7u virus. Mina\u00e7u virus is DCA resistant; haemagglutinating activity was negative. By electronic microscopy non\u2010enveloped virus particles were 75\u2003nm in diameter. PAGE analysis showed Mina\u00e7u virus genome profile with 10 RNA segments. Infected, non\u2010killed animals died 7\u2003days after inoculation. Tissue lesions were observed in all organs, except the lungs. Intense lesions were observed in the CNS and the heart, where neurone and cardiocyte necroses, respectively, were noted. The liver, spleen and kidneys had moderate tissue changes. Viral antigens were more abundant in the CNS and the heart, and absent in the lungs. In conclusion, Mina\u00e7u virus belongs to the family Reoviridae, genus Orbivirus.<\/jats:p>","DOI":"10.1111\/j.1365-2613.2006.00516.x","type":"journal-article","created":{"date-parts":[[2007,1,22]],"date-time":"2007-01-22T10:49:50Z","timestamp":1169462990000},"page":"63-73","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Characterization of Mina\u00e7u virus (Reoviridae: Orbivirus) and pathological changes in experimentally infected newborn mice"],"prefix":"10.1111","volume":"88","author":[{"given":"L\u00edvia C.","family":"Martins","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 A. P.","family":"Diniz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eliana V. P.","family":"Silva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vera L. R. S.","family":"Barros","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hamilton A. O.","family":"Monteiro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raimunda S. S.","family":"Azevedo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juarez A. S.","family":"Quaresma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pedro F. C.","family":"Vasconcelos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,1,22]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"71","article-title":"Estudos sobre a contrasta\u00e7\u00e3o negativa de suspens\u00f5es virais","volume":"44","author":"Barth O.M.","year":"1984","journal-title":"Rev. Bras. Biol."},{"key":"e_1_2_6_3_1","first-page":"797","volume-title":"Diagnostic Procedures for Viral, Rickettsial and Chlamydial Infections","author":"Beaty B.J.","year":"1989"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1958.7.561"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.86.2.490"},{"key":"e_1_2_6_6_1","first-page":"133","article-title":"The use of electron microscopy for diagnosis of virus infections: an overview","volume":"25","author":"Hsiung G.D.","year":"1979","journal-title":"Prog. Med. Virol."},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1177\/29.4.6166661"},{"key":"e_1_2_6_8_1","first-page":"1141","volume-title":"International Catalogue of Arboviruses, including certain other Viruses of Vertebrates","author":"Karabatsos N.","year":"1985"},{"key":"e_1_2_6_9_1","first-page":"137","article-title":"A formaldehyde\u2010glutenaldehyde fixative of high osmolarity for use in electron microscopy","volume":"27","author":"Karnovsky M.J.","year":"1965","journal-title":"J. Cell Biol."},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.9.2.409"},{"key":"e_1_2_6_12_1","first-page":"295","volume-title":"T\u00e9cnica histol\u00f3gica em anatomia patol\u00f3gica: com instru\u00e7\u00f5es para cirurgi\u00e3o, enfermeira e citot\u00e9cnico","author":"Michalany J.","year":"1998"},{"key":"e_1_2_6_13_1","first-page":"169","volume-title":"Tratado de Infectologia","author":"Pinheiro F.P.","year":"1996"},{"key":"e_1_2_6_14_1","first-page":"80","volume-title":"Laboratory Methods in Histotechnology","author":"Prophet E.B.","year":"1992"},{"key":"e_1_2_6_15_1","first-page":"493","article-title":"A simple method of estimating fifty per cent end\u2010points","volume":"27","author":"Reed L.J.","year":"1938","journal-title":"Am. J. Hyg."},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.17.1.208"},{"key":"e_1_2_6_17_1","first-page":"1275","volume-title":"Virology","author":"Schiff L.A.","year":"1990"},{"key":"e_1_2_6_18_1","doi-asserted-by":"crossref","first-page":"1053","DOI":"10.4269\/ajtmh.1979.28.1053","article-title":"A method for the isolation and identification of dengue viruses, using mosquito cell cultures","volume":"28","author":"Tesh R.B.","year":"1979","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.3181\/00379727-96-23483"},{"key":"e_1_2_6_20_1","first-page":"207","volume-title":"Doen\u00e7as Infecciosas e Parasit\u00e1rias \u2013 Enfoque Amaz\u00f4nico","author":"Travassos da Rosa A.P.A.","year":"1997"},{"key":"e_1_2_6_21_1","first-page":"1307","volume-title":"Virology","author":"Tyler K.L.","year":"1990"},{"key":"e_1_2_6_22_1","first-page":"1167","volume-title":"Virus Taxonomy. VII report of the ICTV","author":"Van Regenmortel M.H.V.","year":"2000"},{"key":"e_1_2_6_23_1","first-page":"117","article-title":"Clinical and ecoepidemiological situation of human arboviruses in Brazilian Amazon","volume":"44","author":"Vasconcelos P.F.C.","year":"1992","journal-title":"Braz. J. Adv. Sci."},{"key":"e_1_2_6_24_1","first-page":"72","volume-title":"An Overview of Arbovirology in Brazil and Neighbouring Countries","author":"Vasconcelos P.F.C.","year":"1998"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid0704.017420"}],"container-title":["International Journal of Experimental Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1365-2613.2006.00516.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1365-2613.2006.00516.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,12]],"date-time":"2023-10-12T23:06:32Z","timestamp":1697151992000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1365-2613.2006.00516.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,1,22]]},"references-count":24,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2007,2]]}},"alternative-id":["10.1111\/j.1365-2613.2006.00516.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1365-2613.2006.00516.x","archive":["Portico"],"relation":{},"ISSN":["0959-9673","1365-2613"],"issn-type":[{"value":"0959-9673","type":"print"},{"value":"1365-2613","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,1,22]]},"assertion":[{"value":"2007-01-22","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}