{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,3]],"date-time":"2025-06-03T17:44:54Z","timestamp":1748972694420},"reference-count":35,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2007,3,7]],"date-time":"2007-03-07T00:00:00Z","timestamp":1173225600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Experimental Path"],"published-print":{"date-parts":[[2007,6]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Murine leprosy is a natural disease of the mouse, the most popular model animal used in biomedical research; the disease is caused by <jats:italic>Mycobacterium lepraemurium<\/jats:italic> (MLM), a successful parasite of macrophages. The aim of the study was to test the hypothesis that MLM survives within macrophages because it highly resists the toxic effects of the reactive oxygen intermediaries produced by these cells in response to infection by the microorganism. MLM cells were incubated in the presence of horseradish peroxidase (HRPO)\u2013H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>\u2013halide for several periods of time. The peroxidative effect of this system was investigated by assessing the changes occurred in (a) lipid composition; (b) viability; and (c) infectivity of the microorganism. Changes in the lipid composition of peroxidated\u2010 <jats:italic>vs.<\/jats:italic> intact\u2010MLM were analysed by thin layer chromatography. The effect of the peroxidative system on the viability and infectivity of MLM was measured by the alamar blue reduction assay and by its ability to produce an infection in the mouse, respectively. Peroxidation of MLM produced drastic changes in the lipid envelope of the microorganism, killed the bacteria and abolished their ability to produce an <jats:italic>in vivo<\/jats:italic> infection in the mouse. <jats:italic>In vitro<\/jats:italic>, MLM is highly susceptible to the noxious effects of the HRPO\u2013H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>\u2013halide system. Although the lipid envelope of MLM might protect the microorganism from the peroxidative substances produced at \u2018physiological\u2019 concentrations <jats:italic>in vivo<\/jats:italic>, the success of MLM as a parasite of macrophages might rather obey for other reasons. The ability of MLM to enter macrophages without triggering these cells\u2019 oxidative response and the lack of granular MPO in mature macrophages might better explain its success as an intracellular parasite of these cells.<\/jats:p>","DOI":"10.1111\/j.1365-2613.2007.00524.x","type":"journal-article","created":{"date-parts":[[2007,3,7]],"date-time":"2007-03-07T19:19:21Z","timestamp":1173295161000},"page":"137-145","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Effect of reactive oxygen intermediaries on the viability and infectivity of <i>Mycobacterium lepraemurium<\/i>"],"prefix":"10.1111","volume":"88","author":[{"given":"Kendy","family":"Wek\u2010Rodriguez","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mayra","family":"Silva\u2010Miranda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patricia","family":"Arce\u2010Paredes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oscar","family":"Rojas\u2010Espinosa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,3,13]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"830","volume":"55","author":"Baohong J.L.","year":"1987","journal-title":"Drug susceptibility testing of Mycobacterium leprae"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.45.6.1934-1936.2001"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.8.4149-4152.1999"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/156.6.985"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.41.5.1004"},{"key":"e_1_2_6_7_1","volume-title":"Purification of Mycobacterium leprae","author":"Draper P.","year":"1980"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/228860a0"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-83-2-431"},{"key":"e_1_2_6_10_1","first-page":"301","article-title":"Drug resistant\u2010Mycobacterium leprae\u2014results of mouse footpad studies from a laboratory in south India","volume":"74","author":"Ebenezer G.J.","year":"2002","journal-title":"Indian J. 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