{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T14:26:41Z","timestamp":1782743201171,"version":"3.54.5"},"reference-count":56,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2007,7,20]],"date-time":"2007-07-20T00:00:00Z","timestamp":1184889600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Experimental Path"],"published-print":{"date-parts":[[2007,12]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>The genes that mediate fibroproliferative lung disease remain to be defined. Prior studies from our laboratory showed by <jats:italic>in situ<\/jats:italic> hybridization and immunohistochemistry that the genes coding for tumour necrosis factor alpha, transforming growth factor beta, the platelet\u2010derived growth factor A and B isoforms, and alpha\u20101 pro\u2010collagen are expressed in fibroproliferative lesions that develop quickly after asbestos inhalation. These five genes, along with matrix metalloproteinase 9, a collagenase found to be increased in several lung diseases, are known to control matrix production and cell proliferation in humans and animals. Here we show by laser capture microdissection that (i) The six genes are expressed at significantly higher levels in the asbestos\u2010exposed mice when comparing the same anatomic regions \u2018captured\u2019 in unexposed mice. (ii) The bronchiolar\u2010alveolar duct (BAD) junctions, where the greatest number of fibres initially deposit, were always significantly higher than the other anatomic regions for each gene. The first alveolar duct bifurcation (ADB) generally was higher than the second ADB, the ADBs were always significantly higher than the airway walls and pleura, and the airway walls and pleura were generally higher than the unexposed tissues. (iii) Animals exposed for 3\u2003days always exhibited significantly higher levels of gene expression at the BAD junctions and ADBs than animals exposed for 2\u2003days. To our knowledge, this is the first demonstration of a dose\u2013response to a toxic particle <jats:italic>in situ<\/jats:italic>, and this response appears to be dependent on the number of fibres that deposits at the individual anatomic site.<\/jats:p>","DOI":"10.1111\/j.1365-2613.2007.00545.x","type":"journal-article","created":{"date-parts":[[2007,7,21]],"date-time":"2007-07-21T04:02:27Z","timestamp":1184990547000},"page":"415-425","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Laser capture microdissection reveals dose\u2013response of gene expression in situ consequent to asbestos exposure"],"prefix":"10.1111","volume":"88","author":[{"given":"Qi","family":"Yin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arnold R.","family":"Brody","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deborah E.","family":"Sullivan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2007,7,20]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/rr162"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2002-0166TR"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1378\/chest.125.2.754"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.03.00014703"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb.25.3.4466"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)65332-1"},{"issue":"5","key":"e_1_2_6_8_1","first-page":"1147","article-title":"Crocidolite asbestos induces apoptosis of pleural mesothelial cells: role of reactive oxygen species and poly(ADP\u2010ribosyl) polymerase","volume":"105","author":"Broaddus V.C.","year":"1997","journal-title":"Environ. 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