{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T07:12:26Z","timestamp":1778915546773,"version":"3.51.4"},"reference-count":39,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2011,6,11]],"date-time":"2011-06-11T00:00:00Z","timestamp":1307750400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Experimental Path"],"published-print":{"date-parts":[[2011,10]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Oesophageal exposure to duodenogastro\u2010oesophageal refluxate leads to reflux oesophagitis and is implicated in the development of Barrett\u2019s metaplasia (BM). NF\u2010\u03baB signalling in epithelial cells is associated with the activation of transcription factors believed to be central to BM development, whilst NF\u2010\u03baB activation in fibroblasts plays a critical role in matrix remodelling. Our aim was to study the effects of acid exposure on NF\u2010\u03baB activation in primary human oesophageal fibroblasts (HOFs) and primary and immortalized oesophageal squames and to investigate any epithelial\/stromal interactions in the response of these cells to acid. Primary HOFs and primary and immortalized oesophageal epithelial cells were exposed to acid (pH 7 \u2013 pH 4\u2003\u2264\u2003120\u2003min) in single or pulsed treatments. Conditioned medium from epithelial cells following acid exposure was also applied to fibroblasts. Cell viability was determined by MTT\u2010ESTA. NF\u2010\u03baB activation was determined by cellular localization of NF\u2010\u03baB\/p65 visualized by immunofluorescence. Conditioned medium from oesophageal epithelial cells, subjected to pH 5 pulsatile exposure, activated NF\u2010\u03baB in fibroblasts, with some inter\u2010patient variability, but these conditions did not directly activate NF\u2010\u03baB in the epithelial cells themselves. Significant NF\u2010\u03baB activation was seen in the epithelial cells but only with greater acidity and exposure times (pH 4, 60\u2013120\u2003min). Our findings show that acid exposure can cause indirect activation of stromal cells by epithelial\u2013stromal interactions. This may contribute to the pathogenesis of oesophageal diseases, and the inter\u2010patient variability may go some way to explain why some patients with reflux oesophagitis develop BM and others do not.<\/jats:p>","DOI":"10.1111\/j.1365-2613.2011.00778.x","type":"journal-article","created":{"date-parts":[[2011,6,11]],"date-time":"2011-06-11T06:15:36Z","timestamp":1307772936000},"page":"345-356","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["NF\u2010\u03baB is activated in oesophageal fibroblasts in response to a paracrine signal generated by acid\u2010exposed primary oesophageal squamous cells"],"prefix":"10.1111","volume":"92","author":[{"given":"Nicola H.","family":"Green","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qizhi","family":"Huang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bernard M.","family":"Corfe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jonathan P.","family":"Bury","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sheila","family":"MacNeil","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,6,11]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.sla.0000118751.95179.c6"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1442-2050.2005.00497.x"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.iy.12.040194.001041"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.274.5288.782"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.22742"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2005.12.032"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/bgi211"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1442-2050.2006.00596.x"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s005350300001"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1572-0241.2006.01005.x"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M212714200"},{"issue":"5","key":"e_1_2_8_13_1","doi-asserted-by":"crossref","first-page":"663","DOI":"10.1242\/jcs.110.5.663","article-title":"Acid modulation of HT29 cell growth and differentiation. 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