{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,13]],"date-time":"2026-09-13T23:49:35Z","timestamp":1789343375887,"version":"build-2803163510"},"reference-count":38,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,12,8]],"date-time":"2005-12-08T00:00:00Z","timestamp":1134000000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Paed Dentistry"],"published-print":{"date-parts":[[2006,1]]},"abstract":"<jats:p><jats:bold>Summary.<\/jats:bold><jats:bold> Aim.\u2002<\/jats:bold> The aim of this study was to determine whether there are any differences in the number and distribution of immune cells within human primary and permanent tooth pulp, both in health and disease.<\/jats:p><jats:p><jats:bold>Design.\u2002<\/jats:bold> The research took the form of a quantitative immunocytochemical study.<\/jats:p><jats:p>One hundred and twenty\u2010four mandibular first permanent molars and second primary molars were obtained from children requiring dental extractions under general anaesthesia. Following exodontia, 10\u2010\u00b5m\u2010thick frozen pulp sections were processed for indirect immunofluorescence. Triple\u2010labelling regimes were employed using combinations of the following: (1) protein gene product 9\u00b75, a general neuronal marker; (2) leucocyte common antigen (LCA); and (3) <jats:italic>Ulex europaeus<\/jats:italic> I lectin, a marker of vascular endothelium. Image analysis was then used to determine the percentage area of immunostaining for LCA.<\/jats:p><jats:p><jats:bold>Results.\u2002<\/jats:bold> Leucocytes were significantly more abundant in the pulp horn and mid\u2010coronal region of intact and carious primary teeth, as compared to permanent teeth (<jats:italic>P\u00a0&lt;<\/jats:italic>\u00a00\u00b705, <jats:sc>anova<\/jats:sc>). Both dentitions demonstrated the presence of well\u2010localized inflammatory cell infiltrates and marked aborization of pulpal nerves in areas of dense leucocyte accumulation.<\/jats:p><jats:p><jats:bold>Conclusions.\u2002<\/jats:bold> Primary and permanent tooth pulps appear to have a similar potential to mount inflammatory responses to gross caries The management of the compromised primary tooth pulp needs to be reappraised in the light of these findings.<\/jats:p>","DOI":"10.1111\/j.1365-263x.2006.00682.x","type":"journal-article","created":{"date-parts":[[2005,12,8]],"date-time":"2005-12-08T14:40:18Z","timestamp":1134052818000},"page":"2-9","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Immunocytochemical investigation of immune cells within human primary and permanent tooth pulp"],"prefix":"10.1111","volume":"16","author":[{"given":"H. D.","family":"RODD","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F. M.","family":"BOISSONADE","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,12,8]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0099-2399(06)81564-5"},{"key":"e_1_2_5_3_2","first-page":"576","volume-title":"Leucocyte Typing III","author":"Hogg N","year":"1987"},{"key":"e_1_2_5_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0046-8177(85)80229-X"},{"key":"e_1_2_5_5_2","doi-asserted-by":"crossref","first-page":"3910","DOI":"10.4049\/jimmunol.141.11.3910","article-title":"Characterization of CD45 and CD45R monoclonal antibodies using transfected mouse cell lines that express individual human leukocyte common antigens","volume":"141","author":"Streuli M","year":"1988","journal-title":"Journal of Immunology"},{"key":"e_1_2_5_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0030-4220(66)90143-5"},{"key":"e_1_2_5_7_2","first-page":"130","article-title":"Pulpal response to dental caries in human primary teeth","volume":"34","author":"Cohen S","year":"1967","journal-title":"Journal of Dentistry for Children"},{"key":"e_1_2_5_8_2","first-page":"309","article-title":"Dental pulp conditions in relation to carious lesions","volume":"20","author":"Baume LJ","year":"1970","journal-title":"International Dental Journal"},{"key":"e_1_2_5_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0030-4220(81)90060-8"},{"key":"e_1_2_5_10_2","first-page":"372","article-title":"Histopathology of the pulp in primary incisors with deep dentinal caries","volume":"14","author":"Eidelman E","year":"1992","journal-title":"Pediatric Dentistry"},{"key":"e_1_2_5_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0099-2399(88)80103-1"},{"key":"e_1_2_5_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2776(08)60353-3"},{"key":"e_1_2_5_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1988.tb27002.x"},{"key":"e_1_2_5_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1091590113"},{"key":"e_1_2_5_15_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.102.1.49"},{"key":"e_1_2_5_16_2","doi-asserted-by":"publisher","DOI":"10.1097\/00000478-198707000-00006"},{"key":"e_1_2_5_17_2","doi-asserted-by":"publisher","DOI":"10.1177\/00220345010800011601"},{"key":"e_1_2_5_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-9969(02)00012-2"},{"key":"e_1_2_5_19_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0722.2000.00924.x"},{"key":"e_1_2_5_20_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1469-7580.2003.00153.x"},{"key":"e_1_2_5_21_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2591.1980.tb00875.x"},{"key":"e_1_2_5_22_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2591.1981.tb01054.x"},{"key":"e_1_2_5_23_2","first-page":"101","volume-title":"Human History","author":"Stevens A","year":"1997"},{"key":"e_1_2_5_24_2","doi-asserted-by":"publisher","DOI":"10.1177\/00220345870660061101"},{"key":"e_1_2_5_25_2","doi-asserted-by":"publisher","DOI":"10.1679\/aohc.61.1"},{"key":"e_1_2_5_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9969(95)00024-J"},{"key":"e_1_2_5_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-9969(96)00075-1"},{"key":"e_1_2_5_28_2","first-page":"333","article-title":"Dental pain and the histological condition of the pulp","volume":"20","author":"Tyldesley WR","year":"1970","journal-title":"Dental Practitioner"},{"key":"e_1_2_5_29_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2591.1980.tb00834.x"},{"key":"e_1_2_5_30_2","doi-asserted-by":"publisher","DOI":"10.14219\/jada.archive.1956.0145"},{"key":"e_1_2_5_31_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-9657.1987.tb00619.x"},{"key":"e_1_2_5_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0099-2399(06)81568-2"},{"key":"e_1_2_5_33_2","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1092220310"},{"key":"e_1_2_5_34_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0029(19990515\/01)45:4\/5<285::AID-JEMT12>3.0.CO;2-3"},{"key":"e_1_2_5_35_2","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm\/136.6_Pt_2.S39"},{"key":"e_1_2_5_36_2","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1111\/ipd.1991.1.2.67","article-title":"Formocresol toxicity: is there a suitable alternative for pulpotomy of primary molars?","volume":"2","author":"Ketley CE","year":"1991","journal-title":"International Journal of Paediatric Dentistry"},{"key":"e_1_2_5_37_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-263X.2003.00468.x"},{"key":"e_1_2_5_38_2","first-page":"241","article-title":"Indirect pulp treatment: in vivo outcomes of an adhesive resin system vs calcium hydroxide for the protection of the dentin\u2010pulp complex","volume":"24","author":"Falster CA","year":"2002","journal-title":"Pediatric Dentistry"},{"key":"e_1_2_5_39_2","first-page":"214","article-title":"Caries control and other variables associated with the success of primary molar vital pulp therapy","volume":"26","author":"Vij R","year":"2004","journal-title":"Pediatric Dentistry"}],"container-title":["International Journal of Paediatric Dentistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1365-263X.2006.00682.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,12]],"date-time":"2023-10-12T18:20:02Z","timestamp":1697134802000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1365-263X.2006.00682.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,12,8]]},"references-count":38,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2006,1]]}},"alternative-id":["10.1111\/j.1365-263X.2006.00682.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1365-263x.2006.00682.x","archive":["Portico"],"relation":{},"ISSN":["0960-7439","1365-263X"],"issn-type":[{"value":"0960-7439","type":"print"},{"value":"1365-263X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,12,8]]},"assertion":[{"value":"2005-12-08","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}