{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T20:39:29Z","timestamp":1762375169291},"reference-count":28,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2007,3,16]],"date-time":"2007-03-16T00:00:00Z","timestamp":1174003200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Paed Dentistry"],"published-print":{"date-parts":[[2007,7]]},"abstract":"<jats:p><jats:bold>Introduction.\u2002<\/jats:bold> MSX1 gene has a critical role in craniofacial development, the aim of this case\u2013control study is to test the hypothesis that MSX1 mutation contributes to congenital tooth agenesis in Iranians.<\/jats:p><jats:p><jats:bold>Materials and methods.\u2002<\/jats:bold> The study group consisted of 20 affected individuals with tooth agenesis of lower second premolars or upper lateral incisors with mean age of 24.6. The control group consisted of 20 unaffected individuals. DNA was extracted from all 40 individuals; the polymerase chain reaction (PCR) for MSX1 was carried out with Phenol: Chloroform: Isoamylalchol (PCI) extraction method. Ban II restriction digest and agarose gel electrophoresis of the 20 affected individuals verified the presence of mutation in all 20 affected individuals. The unaffected controls did not show any mutation. Statistical analysis performed by the chi\u2010squared method.<\/jats:p><jats:p><jats:bold>Results.\u2002<\/jats:bold> Ban II did not digest PCR product (DNA) in the control group (195\u00a0bp band on electrophoresis gel) but digested the affected allele (106\u00a0bp and 89\u00a0bp bands). There is a statistically significant correlation between tooth agenesis and MSX1 mutation (<jats:italic>P\u00a0&lt;<\/jats:italic>\u00a00.001).<\/jats:p><jats:p><jats:bold>Conclusion.\u2002<\/jats:bold> The results indicated that MSX1 gene mutation contributes to tooth agenesis in Iranian individuals. As the timing of tooth calcification can vary, radiographic finding of congenital tooth agenesis can be confirmed by this molecular method during different dental ages to achieve certainty.<\/jats:p>","DOI":"10.1111\/j.1365-263x.2006.00820.x","type":"journal-article","created":{"date-parts":[[2007,3,16]],"date-time":"2007-03-16T09:11:19Z","timestamp":1174036279000},"page":"254-258","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["The role of MSX1 in tooth agenesis in Iranians"],"prefix":"10.1111","volume":"17","author":[{"given":"MASSOUD","family":"SEIFI","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"BAHRAM","family":"KAZEMI","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"PARISA","family":"GOLKAR","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,3,16]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1053\/od.1998.v114.a87015"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng0896-417"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1177\/154405910208100410"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1353\/hub.2001.0002"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/1096-8628(20000619)92:5<346::AID-AJMG10>3.0.CO;2-A"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00210412"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1086\/321271"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/74155"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1177\/0810129"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1177\/154405910408300913"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80333-5"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-9525(98)01662-X"},{"key":"e_1_2_6_14_2","unstructured":"ParreidtJ.Z\u00e4hlung der Z\u00e4hne und Benennung der verschiedenen Zahnsorten [Teeth numbering and nomination of different dentition series]. 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Universit\u00e4tspoliklinik zu Leipzig Germany1882:10\u201315."},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/2.2.159"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng0494-329"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng0494-348"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.cr.7290185"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.17.5.2920"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1997.8556"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.15.2.861"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.5.1764"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.molbev.a025707"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.122.10.3141"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.19.3.2080"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/0925-4773(96)00503-5"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.3109\/03008209509013701"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M404143200"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1525-142X.2003.03036.x"}],"container-title":["International Journal of Paediatric Dentistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1365-263X.2006.00820.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1365-263X.2006.00820.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,12]],"date-time":"2023-10-12T04:04:35Z","timestamp":1697083475000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1365-263X.2006.00820.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,3,16]]},"references-count":28,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2007,7]]}},"alternative-id":["10.1111\/j.1365-263X.2006.00820.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1365-263x.2006.00820.x","archive":["Portico"],"relation":{},"ISSN":["0960-7439","1365-263X"],"issn-type":[{"value":"0960-7439","type":"print"},{"value":"1365-263X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,3,16]]},"assertion":[{"value":"2007-03-16","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}