{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,31]],"date-time":"2023-10-31T05:41:02Z","timestamp":1698730862636},"reference-count":27,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2011,8,8]],"date-time":"2011-08-08T00:00:00Z","timestamp":1312761600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Paed Dentistry"],"published-print":{"date-parts":[[2012,1]]},"abstract":"<jats:p><jats:italic>International Journal of Paediatric Dentistry 2011; 22: 52\u201359<\/jats:italic><\/jats:p><jats:p><jats:bold>Objective.\u2002<\/jats:bold> Physiological root resorption is a programmed event that takes place in primary teeth leading to elimination of all root structures. The mechanism behind pulp elimination indicates apoptosis, but its pathway has not been well characterised yet. To better understand this event, we evaluated the gene expression of bax, bcl\u20102, caspase\u20103 and caspase\u20108 through real\u2010time polymerase chain reaction (PCR) and immunohistochemistry expression of Caspase\u20108 and Bax in pulps.<\/jats:p><jats:p><jats:bold>Methods.\u2002<\/jats:bold> Samples were split into two groups: pulps from primary teeth with physiological root resorption (<jats:italic>n<\/jats:italic>\u2003=\u200340) and control (<jats:italic>n<\/jats:italic>\u2003=\u200340), pulps from permanent teeth. Samples of each group were split into PCR (<jats:italic>n<\/jats:italic>\u2003=\u200320) and immunohistochemistry (<jats:italic>n<\/jats:italic>\u2003=\u200320).<\/jats:p><jats:p><jats:bold>Results.\u2002<\/jats:bold> Pulps from primary teeth showed a higher caspase\u20103 mRNA level than pulps from permanent teeth. The expression of bax gene was more intense than caspase\u20108 but both did not show difference between groups. The bcl\u20102 mRNA level was incipient and similar between groups. Histopath slides did not show any evidence of inflammatory infiltration, which implies that extrinsic via is not likely to be involved. Immunohistochemistry reaction to Bax and Caspase\u20108 supported PCR results.<\/jats:p><jats:p><jats:bold>Conclusions.\u2002<\/jats:bold> Pulp apoptosis is likely to occur via caspase\u20103 activation through the mitochondrial pathway.<\/jats:p>","DOI":"10.1111\/j.1365-263x.2011.01157.x","type":"journal-article","created":{"date-parts":[[2011,8,8]],"date-time":"2011-08-08T04:32:59Z","timestamp":1312777979000},"page":"52-59","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Caspase\u20103\/caspase\u20108, bax and bcl2 in pulps of human primary teeth with physiological root resorption"],"prefix":"10.1111","volume":"22","author":[{"given":"LUCIANA V.","family":"RODRIGUES","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"HELEN L.","family":"DEL PUERTO","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"JULIANA M. C.","family":"BRANT","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R\u00d4MULO C.","family":"LEITE","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"ANILTON C.","family":"VASCONCELOS","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,8,8]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-263X.2002.00337.x"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1590\/S0103-64402009000300001"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/35037710"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1126903"},{"key":"e_1_2_7_6_2","first-page":"32","article-title":"Caspase function in programmed cell death","volume":"14","author":"Kumar S","year":"2007","journal-title":"Nature"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2003.08.011"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrc883"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-010-0286-x"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-7-85"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0722.2003.00051.x"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1097\/00004770-200004000-00007"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2008.297"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1080\/01926230701320337"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1051\/medsci\/20102610830"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4419-6706-0_8"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4419-6327-7_8"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1327.1998.2520001.x"},{"key":"e_1_2_7_20_2","first-page":"135","article-title":"Oral malodorous compound activates mitochondrial pathway inducing apoptosis in human gingival fibroblasts","volume":"45","author":"Fujirama M","year":"2009","journal-title":"Clin Oral Investig"},{"key":"e_1_2_7_21_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.2008.01189.x"},{"key":"e_1_2_7_22_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2230.2008.03029.x"},{"key":"e_1_2_7_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)00167-7"},{"key":"e_1_2_7_24_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1071553"},{"key":"e_1_2_7_25_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-263X.2008.00963.x"},{"key":"e_1_2_7_26_2","doi-asserted-by":"publisher","DOI":"10.1590\/S1678-77572009000500024"},{"key":"e_1_2_7_27_2","first-page":"221","article-title":"The effect of physiological root resorption on the histological structure of primary tooth pulp","volume":"23","author":"Sari S","year":"1999","journal-title":"J Clin Pediatr Dent"},{"key":"e_1_2_7_28_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-263X.2006.00682.x"}],"container-title":["International Journal of Paediatric Dentistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1365-263X.2011.01157.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1365-263X.2011.01157.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,30]],"date-time":"2023-10-30T04:06:59Z","timestamp":1698638819000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1365-263X.2011.01157.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,8,8]]},"references-count":27,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2012,1]]}},"alternative-id":["10.1111\/j.1365-263X.2011.01157.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1365-263x.2011.01157.x","archive":["Portico"],"relation":{},"ISSN":["0960-7439","1365-263X"],"issn-type":[{"value":"0960-7439","type":"print"},{"value":"1365-263X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,8,8]]},"assertion":[{"value":"2011-08-08","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}