{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,24]],"date-time":"2025-07-24T12:06:49Z","timestamp":1753358809322},"reference-count":26,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2004,7,1]],"date-time":"2004-07-01T00:00:00Z","timestamp":1088640000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2004,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>One hundred and forty\u2010eight wild whitefish, <jats:italic>Coregonus lavaretus<\/jats:italic> (L.), were caught by electrofishing and sampled for virological examination in December 1999 and 2000, during migration from the brackish water feeding grounds to the freshwater spawning grounds, where the whitefish may come into contact with farmed rainbow trout. All samples were examined on cell cultures. No viruses were isolated. Three viral haemorrhagic septicaemia virus (VHSV) isolates of different origin were tested in infection trials by immersion and intraperitoneal (IP) injection, using 1.5\u2003g farmed whitefish: an isolate from wild caught marine fish, a farmed rainbow trout isolate with a suspected marine origin and a classical freshwater isolate. The isolates were highly pathogenic by IP injection where 99\u2013100% of the whitefish died. Using an immersion challenge the rainbow trout isolates were moderately pathogenic with approximately 20% mortality, whereas the marine isolate was virtually non\u2010pathogenic. At the end of the experiment it was possible to isolate VHSV from survivors infected with the marine and suspected marine isolates. Because of the low infection rate in wild whitefish in Denmark, the role of whitefish in the spread of VHSV in Denmark is probably not significant. The experimental studies, however, showed that whitefish are potential carriers of VHSV as they suffer only low mortality after infection but continue to carry virus.<\/jats:p>","DOI":"10.1111\/j.1365-2761.2004.00554.x","type":"journal-article","created":{"date-parts":[[2004,7,2]],"date-time":"2004-07-02T06:27:33Z","timestamp":1088749653000},"page":"401-408","source":"Crossref","is-referenced-by-count":9,"title":["Investigation of wild caught whitefish, <i>Coregonus lavaretus<\/i> (L.), for infection with viral haemorrhagic septicaemia virus (VHSV) and experimental challenge of whitefish with VHSV"],"prefix":"10.1111","volume":"27","author":[{"given":"H F","family":"Skall","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T E","family":"Kj\u00e6r","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N J","family":"Olesen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,7]]},"reference":[{"key":"e_1_2_19_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0450.1985.tb01940.x"},{"key":"e_1_2_19_3_1","doi-asserted-by":"publisher","DOI":"10.3354\/dao052021"},{"key":"e_1_2_19_4_1","doi-asserted-by":"publisher","DOI":"10.3354\/dao030081"},{"key":"e_1_2_19_5_1","volume-title":"Basic Statistics: A Primer for the Biomedical Sciences","author":"Dunn O.J.","year":"1977"},{"key":"e_1_2_19_6_1","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.79820-0"},{"key":"e_1_2_19_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-7836(93)90015-Y"},{"key":"e_1_2_19_8_1","first-page":"131","article-title":"Preparation of fish tissue cultures for virus research","volume":"59","author":"Jensen M.H.","year":"1963","journal-title":"Bulletin de l'office International des Epizooties"},{"key":"e_1_2_19_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1965.tb14292.x"},{"key":"e_1_2_19_10_1","first-page":"436","article-title":"The ulcus\u2010syndrome in cod (Gadus morhua): III. A preliminary virological report","volume":"31","author":"Jensen N.J.","year":"1979","journal-title":"Nordisk Veterin\u00e6rmedicin"},{"key":"e_1_2_19_11_1","first-page":"73","article-title":"Cod ulcus syndrome rhabdovirus is indistinguishable from the Egtved (VHS) virus","volume":"7","author":"J\u00f8rgensen P.E.V.","year":"1987","journal-title":"Bulletin of the European Association of Fish Pathologists"},{"key":"e_1_2_19_12_1","doi-asserted-by":"publisher","DOI":"10.3354\/dao047081"},{"key":"e_1_2_19_13_1","doi-asserted-by":"publisher","DOI":"10.3354\/dao004035"},{"key":"e_1_2_19_14_1","doi-asserted-by":"publisher","DOI":"10.1006\/fsim.1993.1045"},{"key":"e_1_2_19_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0426.1986.tb00660.x"},{"key":"e_1_2_19_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0959-8030(95)00002-X"},{"key":"e_1_2_19_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-1702(99)00062-3"},{"key":"e_1_2_19_18_1","doi-asserted-by":"publisher","DOI":"10.3181\/00379727-135-34976"},{"key":"e_1_2_19_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0426.1998.tb00638.x"},{"key":"e_1_2_19_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0426.1991.tb00525.x"},{"key":"e_1_2_19_21_1","doi-asserted-by":"publisher","DOI":"10.3354\/dao058099"},{"key":"e_1_2_19_22_1","doi-asserted-by":"publisher","DOI":"10.3354\/dao041231"},{"key":"e_1_2_19_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-1702(99)00056-8"},{"key":"e_1_2_19_24_1","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-78-6-1319"},{"key":"e_1_2_19_25_1","first-page":"186","article-title":"Isolation of viral haemorrhagic septicaemia virus (VHSV) from wild Japanese flounder, Paralichthys olivaceus","volume":"20","author":"Takano R.","year":"2000","journal-title":"Bulletin of the European Association of Fish Pathologists"},{"key":"e_1_2_19_26_1","first-page":"3","article-title":"Mass mortality of pilchard and herring associated with viral hemorrhagic septicemia virus in British Columbia, Canada","volume":"27","author":"Traxler G.S.","year":"1999","journal-title":"American Fisheries Society Fish Health Section Newsletter"},{"key":"e_1_2_19_27_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.151.3713.1004"}],"container-title":["Journal of Fish Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1365-2761.2004.00554.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1365-2761.2004.00554.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,12]],"date-time":"2023-10-12T17:44:48Z","timestamp":1697132688000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1365-2761.2004.00554.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,7]]},"references-count":26,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2004,7]]}},"alternative-id":["10.1111\/j.1365-2761.2004.00554.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1365-2761.2004.00554.x","archive":["Portico"],"relation":{},"ISSN":["0140-7775","1365-2761"],"issn-type":[{"value":"0140-7775","type":"print"},{"value":"1365-2761","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,7]]}}}