{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,13]],"date-time":"2023-10-13T08:13:13Z","timestamp":1697184793622},"reference-count":11,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2006,5,5]],"date-time":"2006-05-05T00:00:00Z","timestamp":1146787200000},"content-version":"vor","delay-in-days":4,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2006,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Infectious salmon anaemia is an important disease of Atlantic salmon. One of the current methods of diagnosis is the indirect fluorescent antibody test (IFAT), using a monoclonal antibody specific to the haemagglutinin of the virus. The conformationally dependent nature of this antibody could be a drawback in its usefulness in other tests. This study describes the development and optimization of a polyclonal antiserum against infectious salmon anaemia virus, including a method of separating virus from cell culture components within culture supernatant. The antiserum was subsequently optimized for use in a variety of immunological diagnostic tests, including IFAT and an alkaline phosphatase\u2010based immunoassay, and Western blot.<\/jats:p>","DOI":"10.1111\/j.1365-2761.2006.00720.x","type":"journal-article","created":{"date-parts":[[2006,5,5]],"date-time":"2006-05-05T15:05:20Z","timestamp":1146841520000},"page":"293-300","source":"Crossref","is-referenced-by-count":1,"title":["Rapid development of polyclonal antisera against infectious salmon anaemia virus and its optimization and application as a diagnostic tool"],"prefix":"10.1111","volume":"29","author":[{"given":"S J","family":"McBeath","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L M","family":"Ellis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P F","family":"Cook","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L","family":"Wilson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K L","family":"Urquhart","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I R","family":"Bricknell","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,5,5]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"152","volume-title":"A Colour Atlas of Salmonid Diseases","author":"Bruno D.W.","year":"1996"},{"key":"e_1_2_6_3_1","first-page":"438","article-title":"Propagation of infectious salmon anaemia (ISA) virus in cell culture","volume":"26","author":"Dannevig B.H.","year":"1995","journal-title":"Veterinary Research"},{"key":"e_1_2_6_4_1","first-page":"499","article-title":"Demonstration of infectious salmon anaemia (ISA) viral antigens in cell cultures and tissue sections","volume":"26","author":"Falk K.","year":"1995","journal-title":"Veterinary Research"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.3354\/dao034077"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2007.00901.x"},{"key":"e_1_2_6_7_1","first-page":"212","article-title":"The use of haemadsorption for the isolation of infectious salmon anaemia virus on SHK\u20101 cells from Atlantic salmon (Salmo salar L.) in Scotland","volume":"20","author":"Smail D.","year":"2000","journal-title":"Bulletin of the European Association of Fish Pathologists"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2761.2003.00444.x"},{"key":"e_1_2_6_9_1","first-page":"1","article-title":"Infectious salmon anaemia: epizootiology and pathology","volume":"9","author":"Stagg R.","year":"1999","journal-title":"State Veterinary Journal"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.76.9.4350"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.3354\/dao044183"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2761.2002.00408.x"}],"container-title":["Journal of Fish Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1365-2761.2006.00720.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1365-2761.2006.00720.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,12]],"date-time":"2023-10-12T11:04:07Z","timestamp":1697108647000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1365-2761.2006.00720.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,5]]},"references-count":11,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2006,5]]}},"alternative-id":["10.1111\/j.1365-2761.2006.00720.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1365-2761.2006.00720.x","archive":["Portico"],"relation":{},"ISSN":["0140-7775","1365-2761"],"issn-type":[{"value":"0140-7775","type":"print"},{"value":"1365-2761","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,5]]}}}