{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T22:23:44Z","timestamp":1761863024562},"reference-count":30,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2011,3,8]],"date-time":"2011-03-08T00:00:00Z","timestamp":1299542400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2011,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p> <jats:italic>Flavobacterium psychrophilum<\/jats:italic> isolates, obtained from ayu, <jats:italic>Plecoglossus altivelis<\/jats:italic>, three species of salmonids and two species of cyprinids in Japan, were used in this study. Bacteria were inoculated to serum prepared from ayu or red spotted masu trout (RSMT), <jats:italic>Oncorhynchus masou ishikawae<\/jats:italic>, and incubated at 18\u2003\u00b0C for 24\u2003h. All isolates (<jats:italic>n<\/jats:italic>\u2003=\u200319) from ayu grew well with a 9\u2010 to 116\u2010fold increase of CFU in ayu serum, while CFU decreased markedly in RSMT serum. In contrast, isolates (<jats:italic>n<\/jats:italic>\u2003=\u200317) from fish species other than ayu exhibited no growth in ayu serum, but some isolates from salmonids survived or grew (1.2\u201323.5 fold increase of CFU) in RSMT serum. The isolates that could not survive or grow in ayu and RSMT sera grew well in both heat\u2010inactivated sera of ayu and RSMT. Experimental infection by intraperitoneal injection showed that ayu isolates examined were all pathogenic to ayu but not to RSMT, while none of the isolates from salmonids and cyprinids were pathogenic to ayu but some showed pathogenicity to RSMT. These results indicate that the <jats:italic>in vitro<\/jats:italic> growth ability of <jats:italic>F.\u00a0psychrophilum<\/jats:italic> isolates in fish serum correlates well with their pathogenicity to fish, particularly in ayu.<\/jats:p>","DOI":"10.1111\/j.1365-2761.2011.01245.x","type":"journal-article","created":{"date-parts":[[2011,3,8]],"date-time":"2011-03-08T13:55:42Z","timestamp":1299592542000},"page":"303-310","source":"Crossref","is-referenced-by-count":15,"title":["Growth of <i>Flavobacterium psychrophilum<\/i> in fish serum correlates with 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