{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T06:58:32Z","timestamp":1792133912811,"version":"4.4.0"},"reference-count":59,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2011,7,18]],"date-time":"2011-07-18T00:00:00Z","timestamp":1310947200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2011,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>There is limited information on antibiotic resistance determinants present in bacteria of aquaculture origin in Australia. The presence of integron and other resistance determinants was investigated in 90 <jats:italic>Aeromonas<\/jats:italic> isolates derived from nine freshwater trout farms in Victoria (Australia). Polymerase chain reaction was carried out for the detection of integrase genes <jats:italic>Int1<\/jats:italic>, <jats:italic>Int2<\/jats:italic> and <jats:italic>Int3<\/jats:italic>, gene cassette array, integron\u2010associated <jats:italic>aadA<\/jats:italic>, <jats:italic>sul1<\/jats:italic> and <jats:italic>qac1<\/jats:italic> genes, streptomycin resistance genes <jats:italic>strA\u2010strB<\/jats:italic>, \u03b2\u2010lactamase resistance genes <jats:italic>bla<\/jats:italic><jats:sub>TEM<\/jats:sub> and <jats:italic>bla<\/jats:italic><jats:sub>SHV<\/jats:sub>, and tetracycline resistance genes <jats:italic>tetA\u2010E<\/jats:italic> and <jats:italic>tetM<\/jats:italic>. Clonal analysis was performed by pulsed\u2010field gel electrophoresis (PFGE). Class 1 integrons were detected in 28\/90 (31%) and class 2 and class 3 in none of the strains, <jats:italic>aadA<\/jats:italic> gene in 19\/27 (70%) streptomycin\u2010resistant strains, <jats:italic>sul1<\/jats:italic> in 13\/15 (86.7%) sulphonamide\u2010resistant strains and <jats:italic>qac1<\/jats:italic> gene in 8\/28 (28.6%) integron\u2010bearing strains. Five strains from two different farms carried gene cassettes of 1000\u2003bp each containing the <jats:italic>aadA<\/jats:italic>2 gene and PFGE analysis revealed genetic relatedness. <jats:italic>tetC<\/jats:italic> was detected in all and <jats:italic>tetA<\/jats:italic> in 9\/18 (50%) tetracycline\u2010resistant strains. The <jats:italic>strA\u2010strB, bla<\/jats:italic><jats:sub>TEM<\/jats:sub> or <jats:italic>bla<\/jats:italic><jats:sub>SHV<\/jats:sub> genes were not detected in any of the strains. <jats:italic>Aeromonas<\/jats:italic> spp. carrying integrons and other resistance genes are present in farm\u2010raised fish and sediments even though no antibiotics were licensed for use in Australian aquaculture at the time of the study.<\/jats:p>","DOI":"10.1111\/j.1365-2761.2011.01272.x","type":"journal-article","created":{"date-parts":[[2011,7,18]],"date-time":"2011-07-18T08:07:27Z","timestamp":1310976447000},"page":"589-599","source":"Crossref","is-referenced-by-count":79,"title":["Incidence of class 1 integron and other antibiotic resistance determinants in <i>Aeromonas<\/i> spp. from rainbow trout farms in Australia"],"prefix":"10.1111","volume":"34","author":[{"given":"O L","family":"Ndi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M 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