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However, clues to the possible functions of GPCR101 may yield from information on the distribution of the receptor and the effect of <jats:italic>in vivo<\/jats:italic> manipulation upon the expression level of the receptor. <jats:italic>In situ<\/jats:italic> hybridisation on mouse brain sections revealed GPCR101 expression in a number of nuclei, including the amygdala, lateral parabrachial nucleus and nucleus of the solitary tract, as well as in the arcuate nucleus, posterior hypothalamus and paraventricular nucleus of the hypothalamus. Food\u2010deprivation was found to increase GPCR101 mRNA level in the posterior hypothalamus and amygdala. In obese mice bearing the <jats:italic>ob<\/jats:italic> gene mutation, GPCR101 mRNA level decreased in the posterior hypothalamus and remained unaltered in the amygdala. By contrast, in both nuclei, GPCR101 mRNA level did not change significantly in obese <jats:italic>ob\/ob<\/jats:italic> mice after intraperitoneal injection of leptin or in mice fed with a high fat diet. These data suggest that GPCR101 mRNA expression in the posterior hypothalamus and amygdala is regulated by a factor(s) other than leptin. Dual <jats:italic>in situ<\/jats:italic> hybridisation was used to establish the relationship between GPCR101 and neuropeptides expressed in the hypothalamus. In the arcuate nucleus, GPCR101 mRNA was expressed in approximately half of the population of neurones expressing the mRNA for the anorexigenic neuropeptide, pro\u2010opiomelanocortin, which suggests a potential functional relationship.<\/jats:p>","DOI":"10.1111\/j.1365-2826.2006.01502.x","type":"journal-article","created":{"date-parts":[[2006,11,7]],"date-time":"2006-11-07T13:52:10Z","timestamp":1162907530000},"page":"34-45","source":"Crossref","is-referenced-by-count":23,"title":["G Protein\u2010Coupled Receptor 101 mRNA Expression in the Mouse Brain: Altered Expression in the Posterior Hypothalamus and Amygdala By Energetic Challenges"],"prefix":"10.1111","volume":"19","author":[{"given":"K. 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