{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T17:34:47Z","timestamp":1781804087090,"version":"3.54.5"},"reference-count":50,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2012,3,26]],"date-time":"2012-03-26T00:00:00Z","timestamp":1332720000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Neuroendocrinology"],"published-print":{"date-parts":[[2012,4]]},"abstract":"<jats:p>A growing body of evidence indiates that carbon monoxide (CO) acts as a gas neurotransmitter within the central nervous system. Although CO has been shown to affect neurohypophyseal hormone release in response to osmotic stimuli, the precise sources, targets and mechanisms underlying the actions of CO within the magnocellular neurosecretory system remain largely unknown. In the present study, we combined immunohistochemistry and patch\u2010clamp electrophysiology to study the cellular distribution of the CO\u2010synthase enzyme heme oxygenase type 1 (HO\u20101), as well as the actions of CO on oxytocin (OT) and vasopressin (VP) magnocellular neurosecretory cells (MNCs), in euhydrated (EU) and 48\u2010h water\u2010deprived rats (48WD). Our results show the expression of HO\u20101 immunoreactivity both in OT and VP neurones, as well as in a small proportion of astrocytes, both in supraoptic (SON) and paraventricular (PVN) nuclei. HO\u20101 expression, and its colocalisation with OT and VP neurones within the SON and PVN, was significantly enhanced in 48WD rats. Inhibition of HO activity with chromium mesoporphyrin IX chloride (CrMP; 20\u2003\u03bc<jats:sc>m<\/jats:sc>) resulted in a slight membrane hyperpolarisation in SON neurones from EU rats, without significantly affecting their firing activity. In 48WD rats, on the other hand, CrMP resulted in a more robust membrane hyperpolarisation, significantly decreasing neuronal firing discharge. Taken together, our results indicate that magnocellular SON and PVN neurones express HO\u20101, and that CO acts as an excitatory gas neurotransmitter in this system. Moreover, we found that the expression and actions of CO were enhanced in water\u2010deprived rats, suggesting that the state\u2010dependent up\u2010regulation of the HO\u20101\/CO signalling pathway contributes to enhance MNCs firing activity during an osmotic challenge.<\/jats:p>","DOI":"10.1111\/j.1365-2826.2011.02249.x","type":"journal-article","created":{"date-parts":[[2011,11,7]],"date-time":"2011-11-07T11:34:46Z","timestamp":1320665686000},"page":"653-663","source":"Crossref","is-referenced-by-count":26,"title":["Enhanced Expression of Heme Oxygenase\u20101 and Carbon Monoxide Excitatory Effects in Oxytocin and Vasopressin Neurones During Water Deprivation"],"prefix":"10.1111","volume":"24","author":[{"given":"W. L.","family":"Reis","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V. C.","family":"Biancardi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Son","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Antunes\u2010Rodrigues","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. E.","family":"Stern","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,3,26]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1006\/mcne.1993.1049"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.7678352"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1161\/hh0202.104530"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-0173(97)00032-5"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.12.5364"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-4781(96)00183-2"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1997.00725.x"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(94)90018-3"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/1044-7431(92)90068-D"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/0169-328X(94)00273-H"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2004.6.878"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1042\/BSR20090010"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-5728(99)00112-5"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-5728(98)00028-9"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1985.sp015887"},{"key":"e_1_2_6_17_2","volume-title":"The Rat Brain in Stereotaxic Coordinates","author":"Paxinos G","year":"1997"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2007.134379"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/cne.22256"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2007.133223"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2003.042739"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1210\/en.2004-0702"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2011.207340"},{"key":"e_1_2_6_24_2","doi-asserted-by":"crossref","first-page":"1214","DOI":"10.1016\/S0090-9556(24)15048-9","article-title":"Selective inhibition of heme oxygenase, without inhibition of nitric oxide synthase or soluble guanylyl cyclase, by metalloporphyrins at low concentrations","volume":"27","author":"Appleton SD","year":"1999","journal-title":"Drug Metab Dispos"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1590\/S0100-879X2004000400001"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1186\/1744-9081-6-64"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbiomolbio.2003.11.015"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.2174\/156720509789207985"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuint.2003.07.005"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2009.173435"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2009.10.021"},{"key":"e_1_2_6_32_2","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.111.175810"},{"key":"e_1_2_6_33_2","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00017.2003"},{"key":"e_1_2_6_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(86)90039-4"},{"key":"e_1_2_6_35_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2826.2010.01961.x"},{"key":"e_1_2_6_36_2","doi-asserted-by":"publisher","DOI":"10.1152\/jn.01267.2005"},{"key":"e_1_2_6_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)45138-1"},{"key":"e_1_2_6_38_2","doi-asserted-by":"publisher","DOI":"10.1006\/abbi.2001.2297"},{"key":"e_1_2_6_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(97)01314-0"},{"key":"e_1_2_6_40_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.35.3.800"},{"key":"e_1_2_6_41_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.20.10925"},{"key":"e_1_2_6_42_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.3029864"},{"key":"e_1_2_6_43_2","doi-asserted-by":"publisher","DOI":"10.1007\/s004290100164"},{"key":"e_1_2_6_44_2","doi-asserted-by":"publisher","DOI":"10.1210\/endo.134.3.7509733"},{"key":"e_1_2_6_45_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1566-0702(00)00279-4"},{"key":"e_1_2_6_46_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0361-9230(96)00209-2"},{"key":"e_1_2_6_47_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.2001.280.6.R1815"},{"key":"e_1_2_6_48_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1460-9568.2003.03005.x"},{"key":"e_1_2_6_49_2","doi-asserted-by":"publisher","DOI":"10.1210\/en.133.3.1466"},{"key":"e_1_2_6_50_2","doi-asserted-by":"publisher","DOI":"10.1089\/152308602753666352"},{"key":"e_1_2_6_51_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00144a001"}],"container-title":["Journal of Neuroendocrinology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1365-2826.2011.02249.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1365-2826.2011.02249.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,13]],"date-time":"2025-03-13T21:22:04Z","timestamp":1741900924000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1365-2826.2011.02249.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,3,26]]},"references-count":50,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2012,4]]}},"alternative-id":["10.1111\/j.1365-2826.2011.02249.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1365-2826.2011.02249.x","archive":["Portico"],"relation":{},"ISSN":["0953-8194","1365-2826"],"issn-type":[{"value":"0953-8194","type":"print"},{"value":"1365-2826","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,3,26]]}}}