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This effect is mediated in part via the corticotrophin\u2010releasing factor (CRF) system, although another potential mechanism is via GABAergic signalling within the medial preoptic area (mPOA) because this has known inhibitory influences on the GnRH pulse generator and shows increased activity during stress. In the present study, we investigated the role of the preoptic endogenous GABAergic system in stress\u2010induced suppression of the GnRH pulse generator. Ovariectomised oestradiol\u2010replaced rats were implanted with bilateral and unilateral cannulae targeting toward the mPOA and lateral cerebral ventricle, respectively; blood samples (25\u2003\u03bcl) were taken via chronically implanted cardiac catheters every 5\u2003min for 6\u2003h for the measurement of LH pulses. Intra\u2010mPOA administration of the specific GABA<jats:sub>A<\/jats:sub>receptor antagonist, bicuculline (0.2\u2003pmol each side, three times at 20\u2010min intervals) markedly attenuated the inhibitory effect of lipopolysaccharide (LPS; 25\u2003\u03bcg\/kg i.v.) but not restraint (1\u2003h) stress on pulsatile LH secretion. By contrast, restraint but not LPS stress\u2010induced suppression of LH pulse frequency was reversed by application of the selective GABA<jats:sub>B<\/jats:sub>receptor antagonist, CGP\u201035348, into the mPOA (1.5\u2003nmol each side, three times at 20\u2010min intervals). However, intra\u2010mPOA application of either bicuculline or CGP\u201035348 attenuated the inhibitory effect of CRF (1\u2003nmol i.c.v.) on the pulsatile LH secretion. These data indicate a pivotal and differential role of endogenous GABAergic signalling in the mPOA with respect to mediating psychological and immunological stress\u2010induced suppression of the GnRH pulse generator.<\/jats:p>","DOI":"10.1111\/j.1365-2826.2011.02270.x","type":"journal-article","created":{"date-parts":[[2011,12,15]],"date-time":"2011-12-15T08:32:44Z","timestamp":1323937964000},"page":"477-488","source":"Crossref","is-referenced-by-count":16,"title":["The Role of GABAergic Signalling in Stress\u2010Induced Suppression of Gonadotrophin\u2010Releasing Hormone Pulse Generator Frequency in Female Rats"],"prefix":"10.1111","volume":"24","author":[{"given":"Y. S.","family":"Lin","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"X. 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